脱氧核糖核酸连接酶在从肺炎球菌中提取的脱氧核糖核酸膜组分中的作用。
Role of deoxyribonucleic acid ligase in a doxyribonucleic acid membrane fraction extracted from pneumococci.
作者信息
Greene M, Firshein W
出版信息
J Bacteriol. 1976 May;126(2):777-84. doi: 10.1128/jb.126.2.777-784.1976.
Deoxyribonucleic acid (DNA) ligase has been detected in a DNA membrane fraction extracted from Pneumococcus. The specific activity of the enzyme in this fraction is 10-fold greater than in the remaining cell extract. It remains firmly bound (with other enzymes) to the complex after a purification procedure in which a considerable percentage of the macromolecules are dissociated. The ligase acts in two ways in the DNA membrane fraction in vitro. One, it catalyzes the linkage of small-molecular-weight pieces of newly synthesized DNA into heavier-molecular-weight DNA strands as shown by others (M Gellert, 1976; R. Okazaki, A. Sugino, S. Hirose, T. Okazaki, Y. Imae, R. Kainuma-Kuroda, T. Ogawa, M. Arisawa, and Y. Kurosowa, 1973; B. Olivera and I. Lehman, 14; and A. Sugino, S. Hirose, and R. Okazaki, 1972) and, two, it protects DNA from degradation by deoxyribonucleases. This latter effect is due to a competition between the ability of the nucleases to degrade DNA and the ability of DNA ligase to seal the nicks produced by these degradative enzymes. The ligase acts cooperatively with other enzymes in the DNA membrane fraction to synthesize DNA.
在从肺炎球菌中提取的DNA膜组分中检测到了脱氧核糖核酸（DNA）连接酶。该组分中该酶的比活性比其余细胞提取物中的高10倍。在一个使相当比例的大分子解离的纯化过程后，它（与其他酶一起）仍牢固地结合在复合物上。在体外，连接酶在DNA膜组分中有两种作用方式。其一，它催化新合成的小分子DNA片段连接成更大分子量的DNA链，这已被其他人证明（M·盖勒特，1976年；冈崎令治、杉野昭、广濑秀、冈崎恒、今江洋、黑田圭沼、小川彻、有泽真人、黑泽洋，1973年；B·奥利韦拉和I·莱曼，1974年；以及杉野昭、广濑秀和冈崎令治，1972年）；其二，它保护DNA不被脱氧核糖核酸酶降解。后一种作用是由于核酸酶降解DNA的能力与DNA连接酶封闭这些降解酶产生的切口的能力之间的竞争。连接酶在DNA膜组分中与其他酶协同作用以合成DNA。
Zotero 插件