Billen D, Hellermann G R, Stallions D R
J Bacteriol. 1975 Oct;124(1):585-8. doi: 10.1128/jb.124.1.585-588.1975.
In a toluene-treated mutant of Escherichia coli K-12 having a temperature-sensitive, conditionally lethal mutation in the structural gene for deoxyribonucleic acid (DNA) ligase, an extensive DNA repair synthesis occurred in X-irradiated cells at the nonpermissive temperature, 42 C. At the permissive temperature, 30 C, nearly normal semiconservative synthesis and limited repair synthesis were observed when DNA ligase was activated by the addition of nicotinamide adenine dinucleotide.
在大肠杆菌K-12的一个经甲苯处理的突变体中,其脱氧核糖核酸(DNA)连接酶的结构基因存在温度敏感型的条件致死突变,在42℃的非允许温度下,受X射线照射的细胞中会发生广泛的DNA修复合成。在30℃的允许温度下,当通过添加烟酰胺腺嘌呤二核苷酸激活DNA连接酶时,可观察到近乎正常的半保留合成和有限的修复合成。