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脱氧核糖核酸连接酶在经甲苯处理的大肠杆菌中依赖脱氧核糖核酸聚合酶I的修复合成中的作用

Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli.

作者信息

Billen D, Hellermann G R, Stallions D R

出版信息

J Bacteriol. 1975 Oct;124(1):585-8. doi: 10.1128/jb.124.1.585-588.1975.

Abstract

In a toluene-treated mutant of Escherichia coli K-12 having a temperature-sensitive, conditionally lethal mutation in the structural gene for deoxyribonucleic acid (DNA) ligase, an extensive DNA repair synthesis occurred in X-irradiated cells at the nonpermissive temperature, 42 C. At the permissive temperature, 30 C, nearly normal semiconservative synthesis and limited repair synthesis were observed when DNA ligase was activated by the addition of nicotinamide adenine dinucleotide.

摘要

在大肠杆菌K-12的一个经甲苯处理的突变体中,其脱氧核糖核酸(DNA)连接酶的结构基因存在温度敏感型的条件致死突变,在42℃的非允许温度下,受X射线照射的细胞中会发生广泛的DNA修复合成。在30℃的允许温度下,当通过添加烟酰胺腺嘌呤二核苷酸激活DNA连接酶时,可观察到近乎正常的半保留合成和有限的修复合成。

相似文献

3
Depression by NAD of x-ray-induced repair-type DNA synthesis in toluene-treated Bacillus subtilis.
Biochim Biophys Acta. 1975 Apr 2;383(4):379-87. doi: 10.1016/0005-2787(75)90307-x.

本文引用的文献

1
Enzymatic cleabage and repair of transforming DNA.转化DNA的酶促切割与修复
Proc Natl Acad Sci U S A. 1969 Jan;62(1):289-96. doi: 10.1073/pnas.62.1.289.
6
Linkage map of Escherichia coli strain K-12.大肠杆菌K-12菌株的连锁图谱。
Bacteriol Rev. 1972 Dec;36(4):504-24. doi: 10.1128/br.36.4.504-524.1972.
7
Genetics and function of DNA ligase in Escherichia coli.大肠杆菌中DNA连接酶的遗传学与功能
J Mol Biol. 1973 Jul 15;77(4):531-47. doi: 10.1016/0022-2836(73)90221-0.

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