Le Cam A, Rey J F, Fehlmann M, Kitabgi P, Freychet P
Am J Physiol. 1979 Jun;236(6):E594-602. doi: 10.1152/ajpendo.1979.236.6.E594.
The effect of partial (70%) hepatectomy on amino acid transport was investigated by measuring the uptake of the nonmetabolizable amino acid analogue alpha-amino-[1(-14C]isobutyric acid in hepatocytes freshly isolated from the liver remnant. In hepatocytes from partially hepatectomized rats, both the influx and the efflux of alpha-aminoisobutyric acid were increased. Quantitative analysis of the relationship between the initial rate of uptake and the substrate concentration indicated that a high-affinity component was responsible for the increased rate of amino acid entry in hepatocytes from partially hepatectomized rats. This component could be detected 90 min after operation, but its Vmax increased with time. The high-affinity component was strictly dependent on sodium and had the properties of a pure A system; its mode of energization partly involved cationic transmembrane gradients. The L system of amino acid transport was not affected after partial hepatectomy. The rapid emergence of a high-affinity amino acid transport system in the liver remnant following partial hepatectomy may play an important role in the regulation of liver regeneration.
通过测量从肝残余新鲜分离的肝细胞中不可代谢氨基酸类似物α-氨基-[1(-14C]异丁酸的摄取，研究了部分（70%）肝切除对氨基酸转运的影响。在部分肝切除大鼠的肝细胞中，α-氨基异丁酸的流入和流出均增加。对摄取初始速率与底物浓度之间关系的定量分析表明，高亲和力成分是部分肝切除大鼠肝细胞中氨基酸进入速率增加的原因。该成分在术后90分钟即可检测到，但其Vmax随时间增加。高亲和力成分严格依赖于钠，具有纯A系统的特性；其供能方式部分涉及阳离子跨膜梯度。部分肝切除后氨基酸转运的L系统未受影响。部分肝切除后肝残余中高亲和力氨基酸转运系统的快速出现可能在肝再生调节中起重要作用。
