Bealer S L, Phillips M I, Johnson A K, Schmid P G
Am J Physiol. 1979 Jun;236(6):E610-5. doi: 10.1152/ajpendo.1979.236.6.E610.
Ablation of anteroventral third ventricle (AV3V) periventricular tissue renders animals temporarily adipsic with no compensatory change in urine volume or concentration. The present experiment was designed to determine whether lesions of the AV3V region attenuate vasopressin (AVP) release in response to intraventricular (ivt) injections of angiotensin II (AII), hypertonic NaCl, and phenylephrine during the adipsic period. Blood pressure, urine conductance, and urine flow rate were monitored in awake, unrestrained animals during a continuous intravenous hydrating infusion. Changes in blood pressure and urine parameters were recorded following ivt injections of 100 ng AII, 500 ng AII, 1 microliter 3% NaCl, and 50 microgram phenylephrine. In addition, a radioimmunoassay (RIA) measured AVP following 500 ng AII ivt in lesioned and nonlesioned animals. Antidiuretic and pressor responses to ivt AII were attenuated after AV3V lesions. In addition, RIA analysis showed a significantly smaller concentration of AVP in lesioned animals following AII injections. These data suggest that the AV3V region is important for AVP release in response to central AII and osmotic stimuli.
损毁腹侧前第三脑室（AV3V）室周组织会使动物暂时出现饮水不能，且尿量和尿浓度无代偿性变化。本实验旨在确定在饮水不能期，AV3V区域的损伤是否会减弱血管加压素（AVP）对脑室内（ivt）注射血管紧张素II（AII）、高渗氯化钠和去氧肾上腺素的释放反应。在清醒、不受约束的动物进行持续静脉补液输注期间，监测其血压、尿导率和尿流率。在ivt注射100 ng AII、500 ng AII、1微升3%氯化钠和50微克去氧肾上腺素后，记录血压和尿参数的变化。此外，采用放射免疫分析法（RIA）测定损毁和未损毁动物在ivt注射500 ng AII后的AVP含量。AV3V损伤后，对ivt AII的抗利尿和升压反应减弱。此外，RIA分析显示，注射AII后，损毁动物体内AVP的浓度显著降低。这些数据表明，AV3V区域对于AVP对中枢AII和渗透压刺激的释放反应很重要。
