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用完整的广州管圆线虫证明酸性磷酸酶活性并特别提及其功能。

Acid phosphatase activity demonstrated by intact Angiostrongylus cantonesis with special reference to its function.

作者信息

Maki J, Yanagisawa T

出版信息

Parasitology. 1979 Dec;79(3):417-23. doi: 10.1017/s0031182000053816.

DOI:10.1017/s0031182000053816
PMID:44361
Abstract

Intact Angiostrongylus cantonensis is able to hydrolyse glucose-phosphate esters, mononucleotides and rho-nitrophenyl phosphate as well as beta-glycerophosphate in vitro. Reciprocal inhibition studies suggest that the hydrolysis of such substrates is due to a non-specific phosphomonoesterase. Molybdate ions, which exert no effect on either the uptake of glucose or the production of lactate, inhibit the hydrolysis of glucose-1-phosphate in the external medium and simultaneously lower the production of lactate by the intact worms in vitro.

摘要

完整的广州管圆线虫能够在体外水解葡萄糖磷酸酯、单核苷酸、对硝基苯磷酸酯以及β-甘油磷酸酯。相互抑制研究表明,此类底物的水解是由一种非特异性磷酸单酯酶引起的。钼酸根离子对葡萄糖摄取或乳酸生成均无影响,但能抑制体外培养的完整虫体对外界培养基中葡萄糖-1-磷酸的水解,并同时降低乳酸的生成量。

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