Fostier A, Breton B, Jalabert B
Ann Endocrinol (Paris). 1979 Jan-Feb;40(1):83-4.
Using radioimmunoassays, plasma levels of carp gonadotropin (c-GTH) and oestradiol-17 beta (E2) are measured every day in Carp, during induced spawning (water temperature = 15 degrees C). Ten Carps injected twice intraperitonealy with a total extract of Carp hypophysis (0,6 mg/kg BW at Jo and 5,4 mg/kg BW at J1) are compared to a lot receiving only saline solution. No evolution is detected in that last group when in the experimental one E2 level increases with exogenous c-GTH (see table). Even if E2 levels are low at the end of vitellogenesis, steroidogenic structures remain sensitive to an exogenous hypophysial stimulation. Secretion ability differs within individuals (correlation coefficient between total estimated c-GTH and E2 = 0,15).
使用放射免疫分析法,在诱导产卵期间(水温 = 15摄氏度),每天测量鲤鱼血浆中鲤鱼促性腺激素(c - GTH)和雌二醇 - 17β(E2)的水平。将10尾鲤鱼分两次腹腔注射鲤鱼垂体总提取物(J0时为0.6毫克/千克体重,J1时为5.4毫克/千克体重),并与仅接受盐溶液的一组进行比较。在最后一组中未检测到变化,而在实验组中,E2水平随外源性c - GTH升高(见表)。即使在卵黄生成末期E2水平较低,类固醇生成结构对外源性垂体刺激仍保持敏感。个体之间的分泌能力存在差异(估计的总c - GTH与E2之间的相关系数 = 0.15)。