Pappius H M, Savaki H E, Fieschi C, Rapoport S I, Sokoloff L
Ann Neurol. 1979 Mar;5(3):211-9. doi: 10.1002/ana.410050302.
The effects of osmotic opening of the blood-brain barrier (BBB) on local cerebral glucose utilization (LCGU) were studied in rats with the carbon 14-deoxyglucose method. The BBB was opened to Evans blue dye by unilateral carotid artery perfusion with hypertonic mannitol solution. (14C)-Deoxyglucose was injected 15 minutes or two to three hours later. Osmotic opening of the BBB resulted in focal areas of intense LCGU in affected areas of the perfused hemisphere. In the contralateral hemisphere, glucose utilization was diminished, especially in cerebral cortical areas. The effects were reversible. LCGU was normal in both hemispheres when permeability to Evans blue returned to normal two to three hours after carotid artery perfusion. In 3 of 5 experiments the administration of high doses of intravenous diazepam immediately following carotid artery perfusion with mannitol prevented an increase in LCGU in the perfused hemisphere but did not prevent lowering of LCGU in the contralateral hemisphere. Increased LCGU following osmotic opening of the BBB was not accompanied by a rise in local cerebral blood flow as measured by the iodoantipyrine method. Blood flow was, in fact, significantly decreased.
采用碳-14-脱氧葡萄糖法，在大鼠中研究了血脑屏障（BBB）渗透性开放对局部脑葡萄糖利用（LCGU）的影响。通过用高渗甘露醇溶液单侧颈动脉灌注，使血脑屏障对伊文思蓝染料开放。15分钟或两到三小时后注射（14C）-脱氧葡萄糖。血脑屏障的渗透性开放导致灌注半球受影响区域出现局部脑葡萄糖利用增强的区域。在对侧半球，葡萄糖利用减少，尤其是在大脑皮质区域。这些影响是可逆的。在颈动脉灌注两到三小时后，当对伊文思蓝的通透性恢复正常时，两个半球的局部脑葡萄糖利用均正常。在5个实验中的3个中，在甘露醇颈动脉灌注后立即静脉注射高剂量地西泮，可防止灌注半球局部脑葡萄糖利用增加，但不能防止对侧半球局部脑葡萄糖利用降低。通过碘安替比林法测量，血脑屏障渗透性开放后局部脑葡萄糖利用增加并未伴随局部脑血流量增加。事实上，血流量显著降低。
