Kaya Mehmet, Gulturk Sefa, Elmas Imdat, Kalayci Rivaze, Arican Nadir, Kocyildiz Zuhal Celebi, Kucuk Mutlu, Yorulmaz Hatice, Sivas Ahmet
Department of Physiology, Istanbul Faculty of Medicine, Istanbul University, Capa-34 390 Istanbul, Turkey.
Life Sci. 2004 Nov 26;76(2):201-12. doi: 10.1016/j.lfs.2004.07.012.
The study was performed to evaluate whether magnesium sulfate could alter the degree of disruption of the blood-brain barrier (BBB) caused by hyperosmotic mannitol. Wistar adult female rats were infused with 25% mannitol into the left internal carotid artery. Each animal received intraperitoneally a 300 mg/kg loading dose of magnesium sulfate, dissolved in 0.9% saline, followed by a further 100 mg/kg dose. In the other group, intracarotid infusion of magnesium sulfate was performed at a dose of 150 mg/kg 10 min before mannitol administration. Evans blue (EB) dye was used as a marker of BBB disruption. The measured serum glucose and magnesium levels increased after mannitol and/or magnesium administration when compared with their initial values before treatment (P < 0.01). Water content of the left hemisphere was significantly increased by hyperosmotic mannitol (P < 0.01). The increased water content in the mannitol-perfused hemisphere was significantly decreased by magnesium treatment (P < 0.05). The content of EB dye in the mannitol-perfused hemisphere markedly increased when compared with the right hemisphere of the same brain (P < 0.01). The EB dye content in the mannitol-perfused hemisphere following both intraperitoneal and intraarterial administration of magnesium decreased when compared with mannitol alone (P < 0.01). We conclude that although magnesium sulfate administration by both intracarotid arterial and intraperitoneal routes attenuates BBB disruption caused by hyperosmolar mannitol, particularly intraperitoneal route of magnesium sulfate administration may provide a useful strategy to limit the transient osmotic opening of the BBB.
本研究旨在评估硫酸镁是否能改变高渗甘露醇引起的血脑屏障（BBB）破坏程度。将成年雌性Wistar大鼠的左颈内动脉注入25%甘露醇。每只动物腹腔内注射溶解于0.9%盐水中的300mg/kg负荷剂量硫酸镁，随后再注射100mg/kg剂量。另一组在甘露醇给药前10分钟经颈内动脉注射150mg/kg剂量的硫酸镁。伊文思蓝（EB）染料用作血脑屏障破坏的标志物。与治疗前的初始值相比，甘露醇和/或硫酸镁给药后测得的血清葡萄糖和镁水平升高（P<0.01）。高渗甘露醇使左半球含水量显著增加（P<0.01）。硫酸镁治疗可显著降低甘露醇灌注半球增加的含水量（P<0.05）。与同一大脑的右半球相比，甘露醇灌注半球的EB染料含量明显增加（P<0.01）。与单独使用甘露醇相比，腹腔内和动脉内注射硫酸镁后，甘露醇灌注半球的EB染料含量降低（P<0.01）。我们得出结论，尽管经颈内动脉和腹腔内途径给予硫酸镁均可减轻高渗甘露醇引起的血脑屏障破坏，但特别是腹腔内给予硫酸镁可能是限制血脑屏障短暂渗透性开放的一种有用策略。
