Bozhko G Kh, Shelestin V A
Ukr Biokhim Zh (1978). 1979 Nov-Dec;51(6):615-9.
A complex of chromatin proteins composed of five histon fractions and nine nonhiston fractions and stable in 2M NaCl solution was destructed by treating with urea, changing pH and decreasing the NaCl concentration, No reconstruction of the complex was observed after the said factors has been removed and the initial solvent composition restored. Nevertheless the reconstruction was detected after dissociation of the complex by desalting in the presence of DNA. The reconstruction was successful when stimulated both dimers and tetramers, the chromatin protein complex under study cannot be reconstructed by "self-organization", the only way being through the stage of nucleoproteid formation, and is similar in this respect to some other complex protein structures of the cell.
一种由五种组蛋白组分和九种非组蛋白组分组成、在2M氯化钠溶液中稳定的染色质蛋白复合物，经尿素处理、改变pH值和降低氯化钠浓度后被破坏。在去除上述因素并恢复初始溶剂组成后，未观察到该复合物的重建。然而，在存在DNA的情况下通过脱盐使复合物解离后检测到了重建。当同时刺激二聚体和四聚体时重建成功，所研究的染色质蛋白复合物不能通过“自组装”重建，唯一的途径是通过核蛋白形成阶段，在这方面与细胞的其他一些复杂蛋白质结构相似。
