Barth G, Künkel W
Z Allg Mikrobiol. 1979;19(6):381-90. doi: 10.1002/jobm.3630190603.
In Sm. lipolytica one NAD+-dependent and three NADP+-dependent alcohol dehydrogenases are detectable by polyacrylamide gelelectrophoresis. The NAD+-dependent ADH (ADH I), with a molecular weight of 240,000 daltons, reacts more intensively with long-chain alcohols (octanol) than with short-chain alcohols (methanol, ethanol). The ADH I is not or only minimally subject to glucose repression. Besides the ADH I band no additional inducible NAD+-dependent ADH band is gel-electrophoretically detectable during growth of yeast cells in medium containing ethanol or paraffin. The ADH I band is very probably formed by two ADH enzymes with the same electrophoretic mobility. The NADP+-dependent alcohol dehydrogenases (ADH II--IV) react with methanol, ethanol and octanol with different intensity. In polyacrylamide gradients two bands of NADP+-dependent ADH are detectable: one with a molecular weight of 70,000 daltons and the other with 120,000 daltons. The occurrence of the three NADP+-dependent alcohol dehydrogenases is regulated by the carbon source of the medium. Sm. lipolytica shows a high tolerance against allylalcohol. Resistant mutants can be isolated only at concentrations of 1 M allylalcohol in the medium. All isolates of allylalcohol-resistant mutants show identical growth in medium containing ethanol as the wild type strain.
在解脂耶氏酵母中，通过聚丙烯酰胺凝胶电泳可检测到一种依赖NAD⁺的醇脱氢酶和三种依赖NADP⁺的醇脱氢酶。分子量为240,000道尔顿的依赖NAD⁺的醇脱氢酶（ADH I）与长链醇（辛醇）的反应比与短链醇（甲醇、乙醇）的反应更强烈。ADH I不受或仅受到最小程度的葡萄糖阻遏。在含有乙醇或石蜡的培养基中酵母细胞生长期间，除了ADH I条带外，凝胶电泳未检测到其他可诱导的依赖NAD⁺的醇脱氢酶条带。ADH I条带很可能由两种具有相同电泳迁移率的醇脱氢酶形成。依赖NADP⁺的醇脱氢酶（ADH II - IV）与甲醇、乙醇和辛醇的反应强度不同。在聚丙烯酰胺梯度中可检测到两条依赖NADP⁺的醇脱氢酶条带：一条分子量为70,000道尔顿，另一条为120,000道尔顿。三种依赖NADP⁺的醇脱氢酶的出现受培养基碳源的调节。解脂耶氏酵母对烯丙醇具有高耐受性。仅在培养基中烯丙醇浓度为1 M时才能分离出抗性突变体。所有烯丙醇抗性突变体分离株在含有乙醇的培养基中的生长情况与野生型菌株相同。
