解脂耶氏酵母中酵母氨酸脱氢酶水平的一般及赖氨酸特异性调控

General and lysin specific control of saccharopine dehydrogenase levels in the yeast Saccharomycopsis lipolytica.

作者信息

Gaillardin C M, Poirier L, Ribet A M, Heslot H

出版信息

Biochimie. 1979;61(4):473-82. doi: 10.1016/s0300-9084(79)80203-5.

Abstract

Lysine supplementation of the growth medium of a wild type strain of the yeast Saccharomycopsis lipolytica specifically results in saccharopine dehydrogenase repression. Starvation of the strain for histidine triggers a general depression of various histidine, leucine, arginine and lysine biosynthetic enzymes, including saccharopine dehydrogenase. These two types of control, specific and general, act independently on saccharopine dehydrogenase expression, since mutants which fail to respond to the specific control still are sensitive to the general one. These mutants were first selected as unable to catabolize lysine, suggesting that a link may exist between saccharopine dehydrogenase specific regulation and activity of the catabolic pathway.

摘要

在解脂耶氏酵母野生型菌株的生长培养基中添加赖氨酸会特异性地导致酵母氨酸脱氢酶受到抑制。该菌株缺乏组氨酸会引发多种组氨酸、亮氨酸、精氨酸和赖氨酸生物合成酶（包括酵母氨酸脱氢酶）的普遍抑制。这两种控制类型，即特异性控制和普遍控制，对酵母氨酸脱氢酶的表达独立起作用，因为对特异性控制无反应的突变体对普遍控制仍然敏感。这些突变体最初是作为无法分解代谢赖氨酸而被筛选出来的，这表明酵母氨酸脱氢酶的特异性调节与分解代谢途径的活性之间可能存在联系。