家蝇微粒体组分的UDP-葡萄糖基转移酶活性
UDP-glucosyltransferase activity of housefly microsomal fraction.
作者信息
Morello A, Repetto Y
出版信息
Biochem J. 1979 Mar 1;177(3):809-12. doi: 10.1042/bj1770809.
Abstract
Housefly UDP-glucosyltransferase activity towards p-nitrophenol was demonstrated in a system in vitro. The activity is localized in the microsomal fraction, requires UDP-glucose, is slightly stimulated by Mg2+ and is activated optimally over a wide range of detergent concentration. Phenobarbital increases the enzyme(s) activity about 3-fold, with p-nitrophenol as substrate, which differs from the corresponding mammalian glucuronyltransferase.
摘要
家蝇对对硝基苯酚的UDP-葡萄糖基转移酶活性在体外系统中得到了证实。该活性定位于微粒体部分，需要UDP-葡萄糖，受Mg2+轻微刺激，并且在很宽的去污剂浓度范围内能得到最佳激活。以对硝基苯酚为底物时，苯巴比妥可使该酶活性提高约3倍，这与相应的哺乳动物葡萄糖醛酸基转移酶不同。
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