Avadhani N G
Biochemistry. 1979 Jun 12;18(12):2673-8. doi: 10.1021/bi00579a038.
The mitochondrial poly(adenylic acid)-containing mRNAs in mouse ascites cells pulsed with radioactive precursors contain 35-55 nucleotide long poly(adenylic acid) sequences. These sequences are shortened with age in cells chased with cold medium. The possible relationship between the decay rates of mRNA and their poly(adenylic acid) sequences has been investigated by using mitochondrial RNA and protein synthesis inhibitors. The pattern of mRNA decay as determined by a new solid-phase-bound complementary DNA procedure indicates the presence of two classes of poly(adenylic acid) containing mRNA in mammalian mitochondria: one decaying with a t1/2 of 45 min and the other class with a t1/2 of 210 min. Inhibitors such as ethidium bromide and puromycin which accelerate the decay of mitochondrial mRNA also cause an enhanced decay of poly(adenylic acid) sequences. These results have been interpreted as evidence supporting the involvement of poly(adenylic acid) sequences in the mRNA stability.
用放射性前体脉冲处理的小鼠腹水细胞中的线粒体含聚腺苷酸的mRNA含有35 - 55个核苷酸长的聚腺苷酸序列。在冷培养基中追踪培养的细胞中，这些序列会随着细胞老化而缩短。通过使用线粒体RNA和蛋白质合成抑制剂，研究了mRNA的衰变率与其聚腺苷酸序列之间的可能关系。通过一种新的固相结合互补DNA方法确定的mRNA衰变模式表明，哺乳动物线粒体中存在两类含聚腺苷酸的mRNA：一类的半衰期为45分钟，另一类的半衰期为210分钟。诸如溴化乙锭和嘌呤霉素等加速线粒体mRNA衰变的抑制剂也会导致聚腺苷酸序列的衰变增强。这些结果被解释为支持聚腺苷酸序列参与mRNA稳定性的证据。
