线粒体蛋白质合成：具有真核生物信使核糖核酸特性的核糖核酸。
Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA.
作者信息
Perlman S, Abelson H T, Penman S
出版信息
Proc Natl Acad Sci U S A. 1973 Feb;70(2):350-3. doi: 10.1073/pnas.70.2.350.
Abstract
A heterogeneous RNA fraction with properties resembling those of messenger RNA was identified in mammalian mitochondria. Synthesis of contaminating RNA of nuclear origin was suppressed by treatment with camptothecin. Labeling of the messenger-like RNA is completely inhibited by ethidium bromide, a specific inhibitor of mitochondrial functions.Although mitochondrial protein synthesis resembles that of prokaryotes in several regards, the messenger-like RNA is covalently linked to poly(adenylic acid) [poly(A)]. Poly(A) has thus far been found only in eukaryotic cells. The poly(A) segment has a gel electrophoretic mobility of about 4 S, corresponding to a length of 50-80 nucleotides, and thus resembles in size the poly(A) found in some mammalian viral RNAs. The messenger RNA can be released from the mitochondrial protein-synthesizing structure by treatment with puromycin.
摘要
在哺乳动物线粒体中鉴定出一种具有类似于信使核糖核酸特性的异质核糖核酸组分。用喜树碱处理可抑制核源污染核糖核酸的合成。溴化乙锭是一种线粒体功能的特异性抑制剂，它能完全抑制类信使核糖核酸的标记。尽管线粒体蛋白质合成在几个方面类似于原核生物，但类信使核糖核酸与聚腺苷酸[poly(A)]共价连接。到目前为止，仅在真核细胞中发现了聚腺苷酸。聚腺苷酸片段在凝胶电泳中的迁移率约为4S，对应长度为50 - 80个核苷酸，因此在大小上类似于在一些哺乳动物病毒核糖核酸中发现的聚腺苷酸。用嘌呤霉素处理可使信使核糖核酸从线粒体蛋白质合成结构中释放出来。
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