Beaumont P C, Parsons B J, Phillips G O, Allen J C
Biochim Biophys Acta. 1979 Apr 26;562(2):214-21. doi: 10.1016/0005-2787(79)90167-9.
The extinction coefficients, quantum yields and reactivities of the triplet states of 8-methoxypsoralen and 4,5',8-trimethylpsoralen in methanolic solution have been determined using laser flash photolysis techniques. The second-order rate constants for the quenching of these triplet states by pyrimidine and purine bases were found to be several orders of magnitude lower than those found for other furocoumarin derivatives. This may suggest, therefore, that the skin photosensitising ability of such compounds does not necessarily correlate with in vitro triplet state reactivity. Preliminary experiments on the reactivity of the psoralen triplet state with DNA itself indicate that no transient absorptions due to psoralen excited states can be observed when a photon is absorbed by the psoralen-DNA complex.
利用激光闪光光解技术测定了8-甲氧基补骨脂素和4,5',8-三甲基补骨脂素在甲醇溶液中的三重态的消光系数、量子产率和反应活性。发现嘧啶和嘌呤碱猝灭这些三重态的二级速率常数比其他呋喃香豆素衍生物的二级速率常数低几个数量级。因此，这可能表明这类化合物的皮肤光敏能力不一定与体外三重态反应活性相关。关于补骨脂素三重态与DNA本身反应活性的初步实验表明，当补骨脂素-DNA复合物吸收一个光子时，无法观察到由于补骨脂素激发态引起的瞬态吸收。
