Gupta M, Ali R
J Biochem. 1984 May;95(5):1253-7. doi: 10.1093/oxfordjournals.jbchem.a134729.
The dark interaction of furocoumarins with DNA has been studied by a fluorescence quenching technique. The intrinsic fluorescence of psoralen, 4,5', 8-trimethylpsoralen (TMP) and 8-methoxypsoralen (8-MOP) was quenched to an appreciable extent upon their noncovalent binding to DNA molecule. The analysis of the binding data revealed that TMP binds to DNA with higher efficiency than 8-MOP and psoralen, their apparent Scatchard binding constants being 13.2 X 10(5) M-1, 7.1 X 10(5) M-1, and 12.2 X 10(5) M-1, respectively. The interaction of furocoumarins with DNA was strongly dependent on the conformational stability of DNA in the particular interaction media. The perturbation of DNA structure by changing the ionic environment decreased its interaction with furocoumarins. However, the interaction was facilitated by the presence of an electron-donating moiety in the parent compound, psoralen.
通过荧光猝灭技术研究了呋喃香豆素与DNA的暗相互作用。补骨脂素、4,5',8-三甲基补骨脂素（TMP）和8-甲氧基补骨脂素（8-MOP）在与DNA分子非共价结合后，其固有荧光被显著猝灭。结合数据的分析表明，TMP与DNA的结合效率高于8-MOP和补骨脂素，它们的表观Scatchard结合常数分别为13.2×10⁵ M⁻¹、7.1×10⁵ M⁻¹和12.2×10⁵ M⁻¹。呋喃香豆素与DNA的相互作用强烈依赖于特定相互作用介质中DNA的构象稳定性。通过改变离子环境对DNA结构的扰动降低了其与呋喃香豆素的相互作用。然而，母体化合物补骨脂素中供电子部分的存在促进了这种相互作用。
