van Dijk W, Lasthuis A M, Ferwerda W
Biochim Biophys Acta. 1979 Apr 18;584(1):121-8. doi: 10.1016/0304-4165(79)90242-3.
Tamm-Horsfall glycoprotein preparations were obtained from calf urine by 1.0 M NaCl precipitation followed by 4 M urea/Sepharose 4B chromatography. By using 0.1% sodium dodecyl sulfate polyacrylamide gel electrophoresis a molecular weight of 86 500 +/- 4500 (n = 12) was calculated for the glycoprotein. Amino acid and carbohydrate analyses were performed, the carbohydrate composition being (in residues per 100 amino acid residues in the glycoprotein): fucose, 0.90; galactose, 4.82; mannose, 4.63;N-acetylglucosamine, 7.36; N-acetylgalactosamine, 1.38; sialic acid, 2.93. Under conditions of mild acid hydrolysis (0.05 M H2SO4, 80 degrees C, 1 h) the calf Tamm-Horsfall glycoprotein preparations were degraded partially into two lower molecular weight fragments (approximate Mr 66 000 and 51 000), as shown by polyacrylamide gel electrophoresis, both fragments being periodic acid-Schiff reagent positive.
通过用1.0M氯化钠沉淀，随后进行4M尿素/琼脂糖4B柱色谱法，从小牛尿液中获得了Tamm-Horsfall糖蛋白制剂。通过使用0.1%十二烷基硫酸钠聚丙烯酰胺凝胶电泳，计算出该糖蛋白的分子量为86500±4500（n=12）。进行了氨基酸和碳水化合物分析，碳水化合物组成（以糖蛋白中每100个氨基酸残基中的残基计）为：岩藻糖，0.90；半乳糖，4.82；甘露糖，4.63；N-乙酰葡糖胺，7.36；N-乙酰半乳糖胺，1.38；唾液酸，2.93。在温和酸水解条件下（0.05M硫酸，80℃，1小时），小牛Tamm-Horsfall糖蛋白制剂部分降解为两个较低分子量的片段（约Mr 66000和51000），如聚丙烯酰胺凝胶电泳所示，两个片段均对高碘酸-席夫试剂呈阳性。
