Andersen B R, Harvath L
Biochim Biophys Acta. 1979 Apr 18;584(1):164-73. doi: 10.1016/0304-4165(79)90246-0.
A simple chemical system consisting of FeSO4 and H2O2 (Fenton's reagent) was shown to emit light (chemiluminescence). The addition of tryptophan to the reaction markedly enhanced light production. Very little chemiluminescence was observed when H2O2 was omitted from the reaction and when ferric, instead of ferrous, ions were used. Hydroxyl radical (OH.) and singlet oxygen (1 deltagO2) quenchers suppressed chemiluminescence of the FeSO4 + tryptophan + H2O2 system; and, deuterium oxide (2H2O) enhanced chemiluminescence of both FeSO4 reactions. These observations suggest that a radical chain reaction involving both OH. and 1 deltag O2 is responsible for the chemiluminescent reactions. Six iron-containing proteins, some of which are located within granulocytes, all emitted light in the presence of H2O2. Since iron and H2O2 are present in metabolically stimulated granulocytes, it is likely that chemiluminescent reactions similar to the ones demonstrated in this study account for part of the chemiluminescence of activated granulocytes.
一个由硫酸亚铁（FeSO4）和过氧化氢（H2O2，即芬顿试剂）组成的简单化学体系被证明会发光（化学发光）。向该反应中添加色氨酸会显著增强发光。当反应中省略过氧化氢以及使用铁离子而非亚铁离子时，观察到的化学发光极少。羟基自由基（OH.）和单线态氧（1ΔgO2）猝灭剂会抑制硫酸亚铁 + 色氨酸 + 过氧化氢体系的化学发光；并且，重水（2H2O）会增强硫酸亚铁两个反应的化学发光。这些观察结果表明，涉及OH.和1ΔgO2的自由基链式反应是化学发光反应的原因。六种含铁蛋白质，其中一些位于粒细胞内，在有过氧化氢存在的情况下都会发光。由于铁和过氧化氢存在于代谢活跃的粒细胞中，类似于本研究中所展示的化学发光反应很可能是活化粒细胞化学发光的一部分原因。
