铁-过氧化氢-碘化物细胞毒性系统
The iron-H2O2-iodide cytotoxic system.
作者信息
Klebanoff S J
出版信息
J Exp Med. 1982 Oct 1;156(4):1262-7. doi: 10.1084/jem.156.4.1262.
Abstract
A potent antimicrobial system is described which consists of ferrous sulfate (Fe2+), hydrogen peroxide (H2O2), and iodide in 0.02 M sodium acetate buffer pH 5.5. H2O2 could be replaced by the H2O2-generating system glucose + glucose oxidase. This system, unlike the myeloperoxidase-H2O2-halide system, was ineffective when iodide was replaced by bromide, chloride, or thyroxine, and was inhibited by EDTA, the hydroxyl radical scavengers mannitol and ethanol, and phosphate and lactate buffers at the same concentration and pH as the acetate buffer used. The acetate buffer, however, could be replaced by water. It is proposed that Fe2+ and H2O2 (Fenton's reagent) generate OH X (or a closely related substance), which interacts with iodide to form one or more toxic species.
摘要
描述了一种高效抗菌系统，它由硫酸亚铁（Fe2+）、过氧化氢（H2O2）和碘化物组成，存在于pH 5.5的0.02 M醋酸钠缓冲液中。H2O2可用产生H2O2的系统葡萄糖 + 葡萄糖氧化酶替代。与髓过氧化物酶-H2O2-卤化物系统不同，当碘化物被溴化物、氯化物或甲状腺素替代时，该系统无效，并且会被EDTA、羟基自由基清除剂甘露醇和乙醇以及与所用醋酸钠缓冲液浓度和pH相同的磷酸盐和乳酸盐缓冲液抑制。然而，醋酸钠缓冲液可用水替代。有人提出Fe2+和H2O2（芬顿试剂）会生成OH•（或一种密切相关的物质），它与碘化物相互作用形成一种或多种有毒物质。
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