Martínez-Medellín J, Benavides L
Biochim Biophys Acta. 1979 Apr 18;584(1):84-93. doi: 10.1016/0304-4165(79)90238-1.
Reticulocytes incubated in an isotonic NaCl saline medium containing glucose, glutamine and amino acids, were able to detach both iron atoms from all the transferrin incorporated by them. In the absence of these metabolites, although transferrin uptake was the same, the reticulocytes failed to remove completely the iron from the transferrin which they incorporated. It has been shown before that there is unspecific as well as specific binding of transferrin to the reticulocyte. By incubating the cells in the presence of a high concentration of bovine serum albumin, we have been able to prevent the unsepcific attachment of transferrin. At least 94% of the iodinated transferrin was capable of donating its iron to the reticulocytes.
在含有葡萄糖、谷氨酰胺和氨基酸的等渗氯化钠盐溶液培养基中孵育的网织红细胞，能够从其摄取的所有转铁蛋白中分离出两个铁原子。在缺乏这些代谢物的情况下，尽管转铁蛋白摄取量相同，但网织红细胞无法完全去除它们摄取的转铁蛋白中的铁。此前已表明，转铁蛋白与网织红细胞存在非特异性和特异性结合。通过在高浓度牛血清白蛋白存在的情况下孵育细胞，我们能够防止转铁蛋白的非特异性附着。至少94%的碘化转铁蛋白能够将其铁提供给网织红细胞。
