Donahue S, Struman J A, Gaull G
Am J Pathol. 1974 Nov;77(2):167-3.
Weanling rabbits were injected subcutaneously daily for 35 days with homocysteine thiolactone in an attempt to repeat the results of McCully and Ragsdale. We failed to produce sustained homocyst(e)inemia (or homocysteine thiolactonemia); indeed, transient homocyst(e)inemia could only be demonstrated with a much higher dose of the free base of homocysteine given intraperitoneally. Homocysteine thiolactonemia was observed up to 15 minutes, only after an intravenous injection of homocysteine thiolactone. Analysis of urine and various organs for free amino acids also failed to detect homocyst(e)ine or the thiolactone. Morphologically, we were unable to distinguish between the experimental and control animals.
为了重复麦卡利和拉格斯代尔的实验结果，给断奶幼兔皮下注射高半胱氨酸硫内酯，每日一次，持续35天。我们未能产生持续性高同型半胱氨酸血症（或高半胱氨酸硫内酯血症）；实际上，只有腹腔注射更高剂量的同型半胱氨酸游离碱才能证明有短暂的高同型半胱氨酸血症。仅在静脉注射高半胱氨酸硫内酯后，观察到高半胱氨酸硫内酯血症长达15分钟。对尿液和各种器官进行游离氨基酸分析，也未检测到同型半胱氨酸或硫内酯。在形态学上，我们无法区分实验动物和对照动物。
