Bramley T A
Biochem J. 1974 Jun;140(3):451-60. doi: 10.1042/bj1400451.
Treatment of immature mice with both follicle-stimulating hormone and human chorionic gonadotrophin in vivo resulted in large increases in the specific activities of ovarian alkaline phosphatase and alkaline nucleotidase. The specific activities of other ovarian enzymes studied were not altered by gonadotrophin treatment. A simultaneous change in the Michaelis constant of ovarian alkaline phosphatase accompanied the increase in specific activity. These changes commenced 6-8h after injection of human chorionic gonadotrophin plus follicle-stimulating hormone. Injection of human chorionic gonadotrophin induced the change in Michaelis constant and increased ovarian alkaline phosphatase activity. Treatment with follicle-stimulating hormone had no effect on ovarian alkaline phosphatase. However, follicle-stimulating hormone synergistically augmented the response to human chorionic gonadotrophin. A latent period of about 24h elapsed before this augmentation was expressed. Augmentation of ovarian alkaline phosphatase was directly related to the dose of follicle-stimulating hormone at a fixed dose of chorionic gonadotrophin. No response of ovarian alkaline phosphatase was observed after treatment of immature mice in vivo with oestrogens, progesterone, growth hormone or prolactin. Unlike chorionic gonadotrophin, sheep luteinizing hormone over a wide dose range induced no response within 24h. However, a response in ovarian alkaline phosphatase was observed when sheep luteinizing hormone was administered in combination with follicle-stimulating hormone. The specific activity and K(m) of ovarian alkaline phosphatase increased during normal maturation. The Michaelis constant ceased to increase as sexual maturity was reached. The changes in alkaline phosphatase activity were of a similar magnitude to those induced by gonadotrophin treatment. It is concluded that the changes induced acutely by treatment in vivo with unphysiological doses of gonadotrophins occur in the maturing mouse under the influence of endogenous, homologous gonadotrophins at physiological concentrations.
在体内用促卵泡激素和人绒毛膜促性腺激素处理未成熟小鼠，导致卵巢碱性磷酸酶和碱性核苷酸酶的比活性大幅增加。所研究的其他卵巢酶的比活性未因促性腺激素处理而改变。卵巢碱性磷酸酶米氏常数的同时变化伴随着比活性的增加。这些变化在注射人绒毛膜促性腺激素加促卵泡激素后6 - 8小时开始。注射人绒毛膜促性腺激素诱导了米氏常数的变化并增加了卵巢碱性磷酸酶活性。促卵泡激素处理对卵巢碱性磷酸酶没有影响。然而，促卵泡激素协同增强了对人绒毛膜促性腺激素的反应。在这种增强表现出来之前约有24小时的潜伏期。在固定剂量的绒毛膜促性腺激素下，卵巢碱性磷酸酶的增强与促卵泡激素的剂量直接相关。在用雌激素、孕酮、生长激素或催乳素在体内处理未成熟小鼠后，未观察到卵巢碱性磷酸酶的反应。与绒毛膜促性腺激素不同，在很宽的剂量范围内，绵羊促黄体生成素在24小时内未诱导出反应。然而，当绵羊促黄体生成素与促卵泡激素联合给药时，观察到了卵巢碱性磷酸酶的反应。卵巢碱性磷酸酶的比活性和K(m)在正常成熟过程中增加。随着性成熟的达到，米氏常数停止增加。碱性磷酸酶活性的变化幅度与促性腺激素处理诱导的变化相似。结论是，在生理浓度的内源性同源促性腺激素的影响下，成熟小鼠体内用非生理剂量的促性腺激素进行急性处理所诱导的变化会发生。
