Bramley T A
Biochem J. 1975 Nov;152(2):365-72. doi: 10.1042/bj1520365.
Treatment of mice aged 23-25 days with chorionic gonadotrophin induced large amounts of an ovarian alkaline phosphatase activity (phosphatase Ib) kinetically distinct from that of untreated ovaries (phosphatase I). The activities of alkaline phosphatase I and Ib varied with age in untreated mice. Phosphatase Ib appeared when serum luteinizing hormone concentrations increased (days 4-10 and days 35-45), and disappeared when concentrations were low (days 11-35). Injection of human chorionic gonadotrophin induced progressively larger amounts of phosphatase Ib activity between day 19 and day 29. However, gonadotrophin treatment failed to induce this activity on days 10-18 and 30-35. Nevertheless, during the latter period, human chorionic gonadotrophin induced especially large increases in uterine weight. Treatment at different ages with sheep luteinizing hormone plus human pituitary follicle-stimulating hormone induced a pattern of response identical with that induced by human chorionic gonadotrophin, although sheep luteinizing hormone alone was ineffective before 35 days. In contrast, human luteinizing hormone induced a response in the absence of exogenous follicle-stimulating hormone.
用绒毛膜促性腺激素处理23 - 25日龄的小鼠，可诱导出大量卵巢碱性磷酸酶活性（磷酸酶Ib），其动力学特性与未处理卵巢的碱性磷酸酶（磷酸酶I）不同。在未处理的小鼠中，碱性磷酸酶I和Ib的活性随年龄而变化。磷酸酶Ib在血清促黄体生成素浓度升高时（第4 - 10天和第35 - 45天）出现，在浓度较低时（第11 - 35天）消失。在第19天至第29天期间，注射人绒毛膜促性腺激素可逐渐诱导出更多的磷酸酶Ib活性。然而，在第10 - 18天和第30 - 35天，促性腺激素处理未能诱导出这种活性。尽管如此，在后期，人绒毛膜促性腺激素可使子宫重量显著增加。用绵羊促黄体生成素加人垂体促卵泡激素在不同年龄进行处理，诱导出的反应模式与用人绒毛膜促性腺激素诱导的相同，尽管单独使用绵羊促黄体生成素在35天之前无效。相比之下，人促黄体生成素在没有外源性促卵泡激素的情况下也能诱导出反应。
