Usher D J, Shepherd R J, Deegan T
Thorax. 1974 Nov;29(6):685-9. doi: 10.1136/thx.29.6.685.
, , 685-689. Increases in the serum activities of several enzymes have been reported in patients with asthma. Liver damage, resulting from altered tensions of oxygen and carbon dioxide in the circulation, has been held to be responsible for the majority of this increase, although it has also been suggested that allergic reactions in the lungs might make some contribution. This communication describes the application of a more specific enzyme assay, the serum lactic dehydrogenase (LDH) isoenzyme pattern, to asthma patients in an attempt to elucidate the source of the increase in enzyme activity. Raised activities of two isoenzymes, LDH-3 and LDH-5, comprised the bulk of the increase in total LDH activity; in contrast, the activities of LDH-1 and LDH-2 were virtually unaltered. Analysis of the distribution of isoenzyme activity in lung tissue homogenate, coupled with knowledge of that in liver, suggested that both tissues contributed towards the effects observed. It appeared probable that the increment in LDH-3 activity arose from lung involvement, whereas the major portion of the increment in LDH-5 activity was derived from the liver.
，，685 - 689。据报道，哮喘患者的几种酶的血清活性会升高。循环中氧气和二氧化碳张力改变导致的肝损伤被认为是这种升高的主要原因，尽管也有人认为肺部的过敏反应可能也有一定作用。本通讯描述了一种更特异的酶测定方法——血清乳酸脱氢酶（LDH）同工酶谱——应用于哮喘患者，以试图阐明酶活性升高的来源。两种同工酶LDH - 3和LDH - 5活性升高构成了总LDH活性升高的主要部分；相比之下，LDH - 1和LDH - 2的活性几乎未改变。对肺组织匀浆中同工酶活性分布的分析，结合对肝脏中同工酶活性分布的了解，表明这两种组织都对观察到的效应有贡献。LDH - 3活性的增加似乎源于肺部受累，而LDH - 5活性增加的主要部分则来自肝脏。
