一种改进的抗生物素蛋白检测微量方法。
An improved micro-method for avidin assay.
作者信息
Elo H, Tuohimaa P
出版信息
Biochem J. 1974 Apr;140(1):115-6. doi: 10.1042/bj1400115.
Abstract
A sensitive method for avidin assay was devised. Tritiated biotin is bound to avidin and this complex is then bound to bentonite. Radioactivity is converted into a gas form by combustion and counted in an automatic proportional counter with 55% efficiency and background of 3.7c.p.m. The sensitivity is 1-2ng of avidin.
摘要
设计了一种用于抗生物素蛋白测定的灵敏方法。将氚标记的生物素与抗生物素蛋白结合，然后将该复合物与膨润土结合。通过燃烧将放射性转化为气体形式，并在自动比例计数器中计数，计数效率为55%，本底为3.7计数/分钟。灵敏度为1 - 2纳克抗生物素蛋白。
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引用本文的文献
1
A radioimmunoassay for chicken avidin. Comparison with a [14C]biotin-binding method.鸡抗生物素蛋白的放射免疫分析。与[14C]生物素结合法的比较。
Biochem J. 1978 Nov 1;175(2):685-90. doi: 10.1042/bj1750685.
本文引用的文献
1
The avidin-biotin equilibrium.抗生物素蛋白-生物素平衡
J Biol Chem. 1951 Nov;193(1):125-32.
2
An automatic gas counter for quantitative micro-determinations of tritium in biological material.一种用于生物材料中氚的定量微量测定的自动气体计数器。
Anal Biochem. 1973 Sep;55(1):193-200. doi: 10.1016/0003-2697(73)90303-5.
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Steroid hormone induction of a specific translatable messenger RNA.
Nat New Biol. 1972 Nov 8;240(97):45-8. doi: 10.1038/newbio240045a0.
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