鸡抗生物素蛋白的放射免疫分析。与[14C]生物素结合法的比较。
A radioimmunoassay for chicken avidin. Comparison with a [14C]biotin-binding method.
作者信息
Kulomaa M S, Elo H A, Tuohimaa P J
出版信息
Biochem J. 1978 Nov 1;175(2):685-90. doi: 10.1042/bj1750685.
A double-antibody solid-phase radioimmunoassay for chicken avidin is reported. Avidin was labelled with 125I by the chloramine-T method. The bound and free avidin were separated with a second antibody bound to a solid matrix. In the logit-log scale the standard curve was linear from 1-2 to 100-200ng of avidin/ml. Cross-reaction of ovalbumin was less than 0.015%. Saturation of biotin-binding sites of avidin with an excess of biotin decreased radioimmunoassay values by about 15%. Recovery studies indicated that avidin can be assayed from all chicken tissues studied with radioimmunoassay, whereas the [14C]biotin/bentonite method gave poor recoveries for avidin in the liver and kidney. Radioimmunoassay and the [14C]biotin/bentonite method gave similar concentrations for oviduct avidin.
本文报道了一种用于检测鸡抗生物素蛋白的双抗体固相放射免疫分析法。采用氯胺 - T法用¹²⁵I标记抗生物素蛋白。用结合于固相基质的第二抗体分离结合态和游离态的抗生物素蛋白。在logit - log尺度下，抗生物素蛋白的标准曲线在1 - 2至100 - 200ng/ml范围内呈线性。卵清蛋白的交叉反应小于0.015%。用过量生物素饱和抗生物素蛋白的生物素结合位点可使放射免疫分析值降低约15%。回收率研究表明，用放射免疫分析法可对所研究的所有鸡组织中的抗生物素蛋白进行检测，而[¹⁴C]生物素/膨润土法对肝脏和肾脏中抗生物素蛋白的回收率较低。放射免疫分析法和[¹⁴C]生物素/膨润土法测得的输卵管抗生物素蛋白浓度相似。
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