Masuoka D T, Jonsson G, Finch C E
Brain Res. 1979 Jun 22;169(2):335-41. doi: 10.1016/0006-8993(79)91034-5.
Brains of C57BL/6J mice, aged 4, 8 and 20--29 months, were examined by the Falck-Hillarp histochemical fluorescence technique. Numerous large, intensely fluorescent green to yellow-green spots (LIFS) were observed in the brains of senescent mice. LIFS were generally round to ovoid in shape and ranged in size from about 10 micrometer to about 30 micrometer. Histochemical and pharmacological procedures and spectral analysis indicated that the formaldehyde-induced fluorescence of the LIFS was due to the presence of catecholamines (CA) rather than aging pigment. Their distribution in the brain suggests an association with nerve axons or terminals rather than cell bodies. The number of LIFS in the hypothalamus increased progressively during aging. It is proposed that LIFS may represent age-related, unusual CA accumulation in enlargements proximal to axonal or terminal portions undergoing spontaneous degeneration.
采用福尔克-希拉尔普组织化学荧光技术对4、8和20 - 29月龄的C57BL/6J小鼠的大脑进行了检查。在衰老小鼠的大脑中观察到大量大的、强荧光的绿至黄绿色斑点（LIFS）。LIFS的形状通常为圆形至椭圆形，大小范围约为10微米至约30微米。组织化学和药理学方法以及光谱分析表明，LIFS的甲醛诱导荧光是由于儿茶酚胺（CA）的存在而非衰老色素。它们在大脑中的分布表明与神经轴突或终末有关，而非与细胞体有关。下丘脑LIFS的数量在衰老过程中逐渐增加。有人提出，LIFS可能代表与年龄相关的、在经历自发退化的轴突或终末部分近端膨大处异常的CA积累。
