Litin B S, Grimes W J
Cancer Res. 1979 Jul;39(7 Pt 1):2595-603.
We have compared cell surface proteins and glycoproteins from normal and transformed cells by two-dimensional electrophoresis. The tumor lines studied include viral and spontaneous transformants which cause tumors that either regress, or grow progressively and kill the host. After the cells were labeled, crude membranes were prepared, and their protein components were resolved by two-dimensional electrophoresis. Cells were labeled with either [35S]methionine, [14C]glucosamine, or 125I-lactoperoxidase. This allows comparison of cell-cell differences in surface structure as well as membrane proteins and glycoproteins. We have demonstrated that not all transformed cell lines give a characteristic "transformed" protein pattern. Many of the differences between the normal and transformed cell protein patterns may be related to alterations in the carbohydrate of membrane glycoproteins. A correlation can be made between the patterns obtained for cell lines and the immunogenicity and tumorigenicity of these cells in vivo.
我们通过二维电泳比较了正常细胞和转化细胞的细胞表面蛋白及糖蛋白。所研究的肿瘤细胞系包括病毒转化细胞系和自发转化细胞系，这些细胞系所引发的肿瘤要么会消退，要么会持续生长并导致宿主死亡。细胞经标记后，制备粗制细胞膜，其蛋白质成分通过二维电泳进行分离。细胞用[35S]甲硫氨酸、[14C]葡糖胺或125I-乳过氧化物酶进行标记。这使得能够比较细胞间表面结构以及膜蛋白和糖蛋白的差异。我们已经证明，并非所有转化细胞系都会呈现出特征性的“转化”蛋白图谱。正常细胞和转化细胞蛋白图谱之间的许多差异可能与膜糖蛋白碳水化合物的改变有关。可以在细胞系所获得的图谱与这些细胞在体内的免疫原性和致瘤性之间建立关联。
