Grimes W J, Greegor S
Cancer Res. 1976 Nov;36(11 Pt 1):3905-10.
Carbohydrate compositions of chloroform: methanol-soluble and -insoluble complex polysaccharides have been studied in cell lines derived from BALB/c mice. The cells used in these studies include normal cells, spontaneous and viral transformants that cause tumors that regress, and spontaneous and viral transformants that cause progressively growing tumors that kill immunocompetent BALB/c mice. The carbohydrates were determined by gas-liquid chromatography. Some of the transformed cell lines compared with normal cells have altered carbohydrate compositions, including decreased sialic acid levels and decreased N-acetylgalactosamine in chloroform: methanol-soluble material. Significant decreases in total carbohydrates of chloroform: methanol-soluble material were also observed in some of the transformed cells. However, these changes alone do not predict cancer. Transformed cell lines that cause progressively growing tumors tend to have fewer alterations in carbohydrates of complex polysaccharides than lines causing tumors that regress.
已对源自BALB/c小鼠的细胞系中氯仿：甲醇可溶性和不溶性复合多糖的碳水化合物组成进行了研究。这些研究中使用的细胞包括正常细胞、导致肿瘤消退的自发转化细胞和病毒转化细胞，以及导致免疫活性BALB/c小鼠死亡的进行性生长肿瘤的自发转化细胞和病毒转化细胞。通过气液色谱法测定碳水化合物。与正常细胞相比，一些转化细胞系的碳水化合物组成发生了改变，包括氯仿：甲醇可溶性物质中唾液酸水平降低和N-乙酰半乳糖胺减少。在一些转化细胞中还观察到氯仿：甲醇可溶性物质的总碳水化合物显著减少。然而，仅这些变化并不能预测癌症。导致进行性生长肿瘤的转化细胞系与导致肿瘤消退的细胞系相比，复合多糖碳水化合物的变化往往较少。
