Woodhead A D, Setlow R B, Scully P M
Cancer Res. 1979 Jul;39(7 Pt 1):2698-703.
We have previously reported the development of an extensive invasive growth of the thyroid gland of the gynogenetic teleost, Poecilia formosa (the Amazon molly), following i.p. injection of UV- or gamma-irradiated thyroid cells. This result was surprising by comparison with mammalian work, in which the thyroid is rarely the site for tumor metastases, but the anatomy of the circulation of fish is different from mammals, and in fish the gills and thyroid gland would be among the first tissues in which injected cells might be arrested. Techniques using a fluorescent dye, 125I membrane label, or [3H]thymidine label were used to follow the distribution of i.p. injected cells in the Amazon molly. Fish sampled as soon as 30 min after injection had some labeled cells dispersed in the connective tissue around the ventral aorta and in the bases of the gills, and by 1 to 4 hr large numbers of cells had moved into the thyroid region. A few cells still persisted there 200 hr later. Experiments on the distribution of heat-killed cells indicated that the initial distribution of the cells was largely governed by mechanical factors. Injected cells would appear to be disseminated in fish by mechanisms similar to those in mammals.
我们之前报道过，在腹腔注射紫外线或伽马射线照射过的甲状腺细胞后，雌核发育硬骨鱼——帆鳉（又称亚马逊帆鳉）的甲状腺出现了广泛的侵袭性生长。与哺乳动物的研究结果相比，这一结果令人惊讶，在哺乳动物中甲状腺很少是肿瘤转移的部位，但鱼类的血液循环解剖结构与哺乳动物不同，在鱼类中，鳃和甲状腺可能是注入细胞最先滞留的组织之一。我们使用了荧光染料、125I膜标记或[3H]胸腺嘧啶核苷标记等技术来追踪腹腔注射的细胞在亚马逊帆鳉体内的分布情况。注射后30分钟内取样的鱼，其腹主动脉周围的结缔组织和鳃基部有一些标记细胞分散，到1至4小时时，大量细胞已进入甲状腺区域。200小时后仍有少数细胞留在那里。对热灭活细胞分布的实验表明，细胞的初始分布很大程度上受机械因素控制。注入的细胞在鱼体内的传播机制似乎与哺乳动物中的类似。
