衰老烟草叶片中的亮氨酰转移核糖核酸合成酶
Leucyl-transfer ribonucleic acid synthetase in senescing tobacco leaves.
作者信息
Nathan I, Richmond A
出版信息
Biochem J. 1974 May;140(2):169-73. doi: 10.1042/bj1400169.
Abstract
- The activity of leucyl-tRNA synthetase obtained from tobacco leaves declined by 50% over a period of 4 days senescence induced by detachment. In addition tRNA(Leu) from senescing leaves was charged to a lesser extent than tRNA(Leu) extracted from mature leaves immediately after detachment. 2. tRNA(Leu) was charged with a synthetase preparation from either mature or senescent leaves and chromatographed on an RPC 3 column. The elution profile showed that the marked decline in specific activity of leucyl-tRNA synthetase in senescent leaves was not associated with a loss of acylation of any isoacceptor of tRNA(Leu).
摘要
- 从烟草叶片中提取的亮氨酰 - tRNA合成酶的活性，在离体诱导衰老的4天时间里下降了50%。此外，衰老叶片中的tRNA(Leu)比刚离体后从成熟叶片中提取的tRNA(Leu)被氨酰化的程度更低。2. 将tRNA(Leu)用来自成熟或衰老叶片的合成酶制剂进行氨酰化，并在RPC 3柱上进行色谱分析。洗脱图谱显示，衰老叶片中亮氨酰 - tRNA合成酶比活性的显著下降与tRNA(Leu)任何同功受体的氨酰化丧失无关。
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