来自烟草健康组织和冠瘿组织的同功tRNA的反相色谱分析

Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum.

作者信息

Cornelis P, Classen E, Claessen J

出版信息

Nucleic Acids Res. 1975 Jul;2(7):1153-61. doi: 10.1093/nar/2.7.1153.

DOI:10.1093/nar/2.7.1153

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343502/

Abstract

RPC 5 (Reversed Phase Chromatography) of aminoacyl-tRNA's from healthy and crown gall (induced by Agrobacterium tume-faciens strain B6) tobacco tissues were compared for eleven amino acids. For ten amino acids: alanine, arginine, glutamic acid, glycine, isoleucine, leucine, lysine, methionine, tyrosine, and valine, no qualitative or quantitative differences could be detected between aminoacyl-tRNA's from both sources. Phenylalanyl-tRNA's from crown gall tissues gave two peaks on RPC 5; the minor early eluting species (peak 1) was always absent in elution profiles of phenylalanyl-tRNA's from healthy tissues or from tobacco leaves. After the "Y" base was removed by pH 2.9 treatment, peak 2 of phenylalanine tRNA was shifted to the position of peak 1.

摘要

对来自健康烟草组织以及冠瘿（由根癌土壤杆菌B6菌株诱导产生）烟草组织的氨酰 - tRNA进行了11种氨基酸的反相色谱（RPC 5）分析。对于10种氨基酸：丙氨酸、精氨酸、谷氨酸、甘氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、酪氨酸和缬氨酸，未检测到来自两种来源的氨酰 - tRNA在定性或定量上的差异。来自冠瘿组织的苯丙氨酰 - tRNA在RPC 5上产生两个峰；健康组织或烟草叶片的苯丙氨酰 - tRNA洗脱图谱中始终不存在较小的早期洗脱物种（峰1）。经pH 2.9处理去除“Y”碱基后，苯丙氨酸tRNA的峰2移至峰1的位置。

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