来自患骨生成障碍病雏鸡的盐溶性弹性蛋白。
Salt-soluble elastin from lathyritic chicks.
作者信息
Sykes B C, Partridge S M
出版信息
Biochem J. 1974 Aug;141(2):567-72. doi: 10.1042/bj1410567.
Abstract
The isolation of a salt-soluble homogeneous elastin from the aortas of lathyritic chicks by chromatography on DEAE-cellulose and salt precipitation is described. These new techniques, as well as some previously published by other workers, were evaluated with the help of antiserum raised in sheep against insoluble chick elastin. The purified elastin was very basic and behaved in a predictable manner in coacervation studies. The protein migrated in sodium dodecyl sulphate-polyacrylamide gels as a single band moving slightly faster than pyruvate kinase (mol.wt. 57000).
摘要
本文描述了通过DEAE-纤维素柱层析和盐沉淀法从患骨生成障碍病雏鸡的主动脉中分离出一种盐溶性均一弹性蛋白的方法。借助于用绵羊针对不溶性雏鸡弹性蛋白产生的抗血清，对这些新技术以及其他研究人员先前发表的一些技术进行了评估。纯化后的弹性蛋白具有很强的碱性，并且在凝聚研究中表现出可预测的行为。该蛋白质在十二烷基硫酸钠-聚丙烯酰胺凝胶中迁移时呈现为单一条带，其迁移速度略快于丙酮酸激酶（分子量57000）。
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