可溶性弹性蛋白的分子量和斯托克斯半径。

Molecular weights and Stokes radii of soluble elastins.

作者信息

Partridge S M, Whiting A H

出版信息

Biochim Biophys Acta. 1979 Jan 25;576(1):71-80. doi: 10.1016/0005-2795(79)90485-9.

Abstract

Soluble elastin was isolated from lathyritic chick aorta using neutral salt solutions in the presence of beta-amino propionitrile. The effect of a carboxy-methylation step in conjunction with proteolytic inhibitors was investigated. Hydrodynamic (Stokes) radii of soluble elastins were measured by gel filtration and the molecular size and weight distribution in purified fractions are reported.

摘要

在β-氨基丙腈存在的情况下，使用中性盐溶液从患骨关节炎的雏鸡主动脉中分离出可溶性弹性蛋白。研究了羧甲基化步骤与蛋白水解抑制剂相结合的效果。通过凝胶过滤测量可溶性弹性蛋白的流体动力学（斯托克斯）半径，并报告纯化级分中的分子大小和重量分布。