Shoor P M, Marzec U M, Griffith L D, Hammill F S, Dilley R B, Bernstein E F
Circ Res. 1979 Jul;45(1):100-7. doi: 10.1161/01.res.45.1.100.
To study the influence of arterial pulse pressure on renin release, a chronic pulseless calf preparation was developed using a centrifugal left ventricular bypass blood pump. After pump implantation and recovery, control measurements of renal vein and arterial plasma renin activity, arterial pressure, and renal artery flow were obtained. The centrifugal bypass pump rate then was increased to capture cardiac output completely, and nine conscious calves were perfused in a nonpulsatile manner (pulse pressure less than 5-10 mm Hg) for 48 hours. Nonpulsatile perfusion was well tolerated and serum sodium, potassium, creatinine, and blood urea nitrogen were unchanged during bypass. Mean arterial pressure remained relatively constant [114 +/- 3 (SE) mm Hg] during bypass, and was not significantly changed from control. Although renal blood flow decreased slightly from control (667 +/- 84 ml/min) during the nonpulsatile perfusion period (555 +/- 73 ml/min), renin secretion did not increase significantly from control (482 +/- 81 ng angiotensin I/ml per hr per min) during the bypass period (531 +/- 99). A diurnal cycle of renin secretin was observed during the pulseless perfusions. These data document the lack of any significant stimulatory influence of decreased pulse pressure on renin secretion in a chronic awake calf model.
为研究动脉脉压对肾素释放的影响，使用离心式左心室旁路血泵建立了慢性无脉小牛模型。在植入血泵并恢复后，对肾静脉和动脉血浆肾素活性、动脉压及肾动脉血流量进行了对照测量。然后增加离心旁路血泵的速率以完全捕获心输出量，对9只清醒小牛进行了48小时的非搏动性灌注（脉压小于5 - 10 mmHg）。非搏动性灌注耐受性良好，旁路灌注期间血清钠、钾、肌酐和血尿素氮均未改变。旁路灌注期间平均动脉压保持相对恒定[114±3（标准误）mmHg]，与对照相比无显著变化。尽管在非搏动性灌注期间肾血流量较对照（667±84 ml/min）略有下降（555±73 ml/min），但旁路灌注期间肾素分泌较对照（482±81 ng血管紧张素I/ml每小时每分钟）并未显著增加（531±99）。在无脉灌注期间观察到肾素分泌的昼夜周期。这些数据表明，在慢性清醒小牛模型中，脉压降低对肾素分泌没有任何显著的刺激作用。
