Florence V M, Bevan J A
Circ Res. 1979 Aug;45(2):212-8. doi: 10.1161/01.res.45.2.212.
Endogenous choline acetyltransferase (ChAT) activity and high affinity (HA) 3H-choline uptake can be assayed specifically and used to quantify cholinergic neuronal mechanisms. In general, arteries at the base of the brain in the cat and rabbit were distinguished by high and in the dog by lower ChAT activity and 3H-choline levels of uptake. There was a linear relationship between the mean values of the observations from of the two assays on given cerebral arteries from the three species (r = 0.871,P less than 0.001). Since ChAT activity and HA 3H-choline uptake essentially are confined to cholinergic nerve terminals, our results imply that the Circle of Willis and its main branches in the cat, rabbit, and dog are cholinergically innervated.
内源性胆碱乙酰转移酶（ChAT）活性和高亲和力（HA）3H-胆碱摄取可进行特异性测定，并用于量化胆碱能神经元机制。一般来说，猫和兔脑底部的动脉ChAT活性高，而狗的ChAT活性和3H-胆碱摄取水平较低。对这三个物种给定脑动脉的两种测定结果的平均值之间存在线性关系（r = 0.871，P小于0.001）。由于ChAT活性和HA 3H-胆碱摄取基本上局限于胆碱能神经末梢，我们的结果表明，猫、兔和狗的Willis环及其主要分支接受胆碱能神经支配。
