Isoelectric focusing of acid hydrolases in human fibroblasts and leucocytes.

Lysosomal hydrolases in fibroblasts and leucocytes exhibited the same isoelectric focusing pattern as found in liver. Treatment with neuraminidase had a great effect on the pattern of several glycosidases while treatment with alkaline phosphatase did not significantly change the pattern. These findings are discussed with special reference to the different "uptake" systems that exist for acid hydrolases.