一种定量淀粉酶的方法及其在各种体液检测中的应用。
A method for quantitating amylase and its use in the investigation of various body fluids.
作者信息
Kipps A E, Whitehead P H
出版信息
Ann Clin Biochem. 1974 Nov;11(6):219-23. doi: 10.1177/000456327401100165.
A plate-diffusion technique for the quantitative assay of amylase in body fluids is described which is dependent on the incorporation of a soluble amylopectin-dye complex into agar. The technique correlates well with other established amylase assays, and has a coefficient of variation of 1.45 for amylase levels within the normal range for serum. Normal levels of amylase in serum and urine are reported which agree with those recorded by previous workers, and in addition values of amylase in semen, saliva, and tears are quoted.
本文描述了一种用于定量测定体液中淀粉酶的平板扩散技术，该技术依赖于将可溶性支链淀粉-染料复合物掺入琼脂中。该技术与其他已确立的淀粉酶测定方法相关性良好，对于血清正常范围内的淀粉酶水平，其变异系数为1.45。报告了血清和尿液中淀粉酶的正常水平，与先前研究者记录的结果一致，此外还引用了精液、唾液和泪液中淀粉酶的值。
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