一种在52摄氏度下快速进行的淀粉酶手工检测方法。
A rapid, manual test for amylase at 52C.
作者信息
Creno R J, Wenk R E, Kennedy J W
出版信息
Am J Med Technol. 1976 Sep;42(9):310-7.
A simple modification of an alpha-amylase (E.C. 3.2.1.1.) procedure, using blue starch substrate, permits quantitative results after three minutes of incubation. The procedure, performed on blank, control, and unknown speciemns, takes advantage of the heat stability of the enzyme and provides immediate and reliable data for acutely ill patients. The performance characteristics of the test on serum and urine are described. The method is distinctly advantageous in prividing rapid results for serum amylase, urine amylase, and amylase clearance in patients with suspected pancreatitis.
对使用蓝色淀粉底物的α-淀粉酶（E.C. 3.2.1.1.）检测方法进行简单修改后，孵育三分钟即可获得定量结果。该方法应用于空白、对照和未知样本，利用了酶的热稳定性，可为急症患者提供即时且可靠的数据。文中描述了该检测方法在血清和尿液中的性能特征。对于疑似胰腺炎患者的血清淀粉酶、尿淀粉酶及淀粉酶清除率，该方法在提供快速结果方面具有明显优势。
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