火鸡肝脏黄嘌呤脱氢酶：依赖于功能性活性位点含量的酶的特性。
Turkey liver xanthine dehydrogenase: properties of the enzyme dependent on the content of functional active sites.
作者信息
Cleere W F, O'Regan C, Coughlan M P
出版信息
Biochem J. 1974 Nov;143(2):465-8. doi: 10.1042/bj1430465.
Abstract
Turkey liver xanthine dehydrogenase containing the full complement of molybdenum, flavin and iron-sulphur prosthetic groups is, as normally isolated, a mixture of functional and non-functional enzyme. The latter apparently lacks the cyanolysable persulphide groups essential to the oxidation of xanthine and to interaction with arsenite. These groups are not required for the oxidation of NADH by Methylene Blue. That KI treatment effects a differential release of flavin from xanthine-prereduced and NADH-prereduced enzyme merely reflects the degree of functionality of the preparations used and may not be taken as evidence for non-equivalence of the flavin chromophores.
摘要
正常分离得到的含有完整钼、黄素和铁硫辅基的火鸡肝脏黄嘌呤脱氢酶是一种功能性和非功能性酶的混合物。后者显然缺乏黄嘌呤氧化和与亚砷酸盐相互作用所必需的可被氰化物裂解的过硫化物基团。这些基团对于亚甲蓝氧化NADH并非必需。碘化钾处理导致黄素从黄嘌呤预还原和NADH预还原的酶中差异释放，这仅仅反映了所用制剂的功能程度，不能作为黄素发色团不等同的证据。
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引用本文的文献
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Turkey liver xanthine dehydrogenase. Relation between nicotinamide-adenine dinucleotide oxidoreductase activity and the content of functional enzyme.火鸡肝脏黄嘌呤脱氢酶。烟酰胺腺嘌呤二核苷酸氧化还原酶活性与功能性酶含量之间的关系。
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2
Oxidation--reduction potentials of turkey liver xanthine dehydrogenase and the origins of oxidase and dehydrogenase behaviour in molybdenum-containing hydroxylases.火鸡肝脏黄嘌呤脱氢酶的氧化还原电位以及含钼羟化酶中氧化酶和脱氢酶行为的起源
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Comparison of the molybdenum centres of native and desulpho xanthine oxidase. The nature of the cyanide-labile sulphur atom and the nature of the proton-accepting group.天然型和脱硫型黄嘌呤氧化酶钼中心的比较。氰化物敏感硫原子的性质和质子接受基团的性质。
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本文引用的文献
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Turkey liver xanthine dehydrogenase. Reactivation of the cyanide-inactivated enxyme by sulphide and by selenide.火鸡肝脏黄嘌呤脱氢酶。硫化物和硒化物对氰化物失活酶的重新激活作用。
Biochem J. 1974 Nov;143(2):331-40. doi: 10.1042/bj1430331.
6
Nonequivalence of the flavin adenine dinucleotide moieties of chicken liver xanthine dehydrogenase.鸡肝黄嘌呤脱氢酶中黄素腺嘌呤二核苷酸部分的不等效性。
J Biol Chem. 1972 Apr 10;247(7):2177-82.
7
The resolution of active and inactive xanthine oxidase by affinity chromatography.通过亲和色谱法分离活性和非活性黄嘌呤氧化酶。
J Biol Chem. 1972 Mar 10;247(5):1597-604.
8
Studies on the dissociation of flavin adenine dinucleotide from metalloflavoproteins.
J Biol Chem. 1972 Feb 10;247(3):765-70.
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Properties of xanthine oxidase preparations dependent on the proportions of active and inactivated enzyme.黄嘌呤氧化酶制剂的性质取决于活性酶和失活酶的比例。
Biochim Biophys Acta. 1970 Sep 16;212(3):523-6. doi: 10.1016/0005-2744(70)90264-0.
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The role of molybdenum in xanthine oxidase and related enzymes. Reactivity with cyanide, arsenite, and methanol.钼在黄嘌呤氧化酶及相关酶中的作用。与氰化物、亚砷酸盐和甲醇的反应活性。
J Biol Chem. 1969 May 25;244(10):2658-63.
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