溴化乙锭对双标准核糖均聚物螺旋的稳定作用及对三链螺旋的去稳定作用。
Stablilzation of two-standard ribohomopolymer helices and destabilzation of a three-stranded helix by ethidium bromide.
作者信息
Waring M J
出版信息
Biochem J. 1974 Nov;143(2):483-6. doi: 10.1042/bj1430483.
Abstract
Thermal-denaturation profiles of helical polynucleotides have been measured in the presence of increasing concentrations of ethidium bromide. The poly(A).poly(U) helix is strongly stabilized by binding of ethidium, to much the same extent as is DNA, but the stabilizing effect on poly(I).poly(C) is much smaller. In the poly(A).2poly(U) system the drug selectively destabilizes and eventually destroys the triple helix, leaving only the double-helix-to-coil transition.
摘要
在溴化乙锭浓度不断增加的情况下，已测量了螺旋状多核苷酸的热变性曲线。聚（A）·聚（U）螺旋通过溴化乙锭的结合而得到强烈稳定，其程度与DNA大致相同，但对聚（I）·聚（C）的稳定作用要小得多。在聚（A）·2聚（U）体系中，该药物选择性地使三链螺旋不稳定并最终将其破坏，只留下双链螺旋到卷曲的转变。
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