Jeffreys A, Craig I
Biochem J. 1974 Oct;144(1):161-4. doi: 10.1042/bj1440161.
The proteins synthesized in the mitochondria of mouse and human cells grown in tissue culture were examined by electrophoresis in polyacrylamide gels. The proteins were labelled by incubating the cells in the presence of [(35)S]methionine and an inhibitor of cytoplasmic protein synthesis (emetine or cycloheximide). A detailed comparison between the labelled products of mouse and human mitochondrial protein synthesis was made possible by developing radioautograms after exposure to slab-electrophoresis gels. Patterns obtained for different cell types of the same species were extremely similar, whereas reproducible differences were observed on comparison of the profiles obtained for mouse and human cells. Four human-mouse somatic cell hybrids were examined, and in each one only components corresponding to mouse mitochondrially synthesized proteins were detected.
通过在聚丙烯酰胺凝胶中进行电泳，对在组织培养中生长的小鼠和人类细胞线粒体中合成的蛋白质进行了检测。通过在[(35)S]甲硫氨酸和细胞质蛋白质合成抑制剂（放线菌酮或环己酰亚胺）存在的情况下培养细胞来标记蛋白质。通过在平板电泳凝胶曝光后显影放射自显影片，得以对小鼠和人类线粒体蛋白质合成的标记产物进行详细比较。同一物种不同细胞类型获得的模式极其相似，而在比较小鼠和人类细胞获得的图谱时则观察到可重复的差异。检测了四个人鼠体细胞杂种，在每一个杂种中仅检测到与小鼠线粒体合成蛋白质相对应的成分。
