Yatscoff R W, Aujume L, Freeman K B, Goldstein S
Can J Biochem. 1978 Oct;56(10):939-42. doi: 10.1139/o78-146.
The products of mitochondrial protein synthesis in established cell lines of various mammalian species were labelled with [35S]methionine and their number and apparent molecular weights determined by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis and fluorography. Proteins synthesized by isolated rat liver mitochondria were labelled with [3H]valine and similarly characterized. Each species had a distinctive pattern of from 10 to 13 mitochondrially synthesized proteins with apparent molecular weights between 10,000 and 50,000. No differences were detected in the number or electrophoretic mobility of the mitochondrially synthesized proteins of SV-40-transformed and nontransformed WI-38 cells.
用[35S]甲硫氨酸标记各种哺乳动物物种已建细胞系中线粒体蛋白质合成的产物，并通过十二烷基硫酸钠聚丙烯酰胺平板凝胶电泳和荧光自显影法测定其数量和表观分子量。用[3H]缬氨酸标记分离的大鼠肝线粒体合成的蛋白质，并进行类似的表征。每个物种都有10到13种线粒体合成蛋白质的独特模式，其表观分子量在10000到50000之间。在SV - 40转化和未转化的WI - 38细胞中线粒体合成蛋白质的数量或电泳迁移率未检测到差异。
