Kossorotow A, Wolf H U, Seiler N
Biochem J. 1974 Oct;144(1):21-7. doi: 10.1042/bj1440021.
The effects of putrescene, spermidine and spermine on membrane-bound acetylcholinesterase from human erythrocyte ;ghosts' and the solubilized enzyme of the electric organ of the electric eel were studied by kinetic methods. Measurements were made by using a photometric method which made it possible to record the enzyme reaction in the steady-state phase. Substrate-concentration-dependent activation and inhibition of acetylcholinesterase by polyamines is similar to that by Na(+), K(+), Ca(2+), Mg(2+) and certain quaternary and bisquaternary amines. The kinetics suggest an allosteric reaction mechanism. On the basis of the kinetic results a role for the polyamines as modulators of synaptic acetylcholinesterase is proposed.
采用动力学方法研究了腐胺、亚精胺和精胺对人红细胞膜结合乙酰胆碱酯酶（“血影”）以及电鳗电器官中可溶性乙酰胆碱酯酶的影响。通过一种光度法进行测量，该方法能够记录稳态阶段的酶反应。多胺对乙酰胆碱酯酶的底物浓度依赖性激活和抑制作用与Na⁺、K⁺、Ca²⁺、Mg²⁺以及某些季铵盐和双季铵盐的作用相似。动力学研究表明其反应机制为别构反应。基于动力学结果，提出了多胺作为突触乙酰胆碱酯酶调节剂的作用。
