Barratt E, Buttery P J, Boorman K N
Biochem J. 1974 Nov;144(2):189-98. doi: 10.1042/bj1440189.
Urate synthesis was studied in a perfused chicken liver preparation. The perfused liver had an ATP/ADP ratio of 0.29+/-0.05(6) compared with 0.34+/-0.07(10) in liver obtained from chicks under ether anaesthesia. Lactate/pyruvate ratios were 9.4+/-1.7(5) in the perfused liver and 14.8+/-1.8(5) in the rapidly sampled liver. Urate synthesis was only marginally stimulated by glycine, glutamine, aspartic acid or NH(4)Cl, but significant increases were observed with phosphoribosyl pyrophosphate, aminoimidazolecarboxylic acid riboside, inosine, inosinic acid and xanthine. Urate synthesis from glycine, glutamine, NH(4)Cl, asparagine, alanine, histidine and a mixture of 21 amino acids was obtained on inclusion of insulin in the perfusion medium. Evidence for the inclusion of the carbon of histidine into uric acid was obtained. Aspects of the energy consumption associated with the conversion of excess of amino acid into uric acid are considered.
在灌注的鸡肝制剂中研究了尿酸合成。灌注肝的ATP/ADP比值为0.29±0.05(6)，而乙醚麻醉下雏鸡肝脏的该比值为0.34±0.07(10)。灌注肝的乳酸/丙酮酸比值为9.4±1.7(5)，快速取样肝脏的该比值为14.8±1.8(5)。甘氨酸、谷氨酰胺、天冬氨酸或氯化铵对尿酸合成仅有轻微刺激作用，但磷酸核糖焦磷酸、氨基咪唑羧酸核糖核苷、次黄苷、次黄苷酸和黄嘌呤可显著增加尿酸合成。在灌注培养基中加入胰岛素后，可从甘氨酸、谷氨酰胺、氯化铵、天冬酰胺、丙氨酸、组氨酸和21种氨基酸混合物中合成尿酸。获得了组氨酸碳掺入尿酸的证据。考虑了与过量氨基酸转化为尿酸相关的能量消耗方面。
