• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜铬粒蛋白“空壳”转运儿茶酚胺的稳态动力学

Steady-state kinetics of catecholamine transport by chromaffin-granule "ghosts".

作者信息

Phillips J H

出版信息

Biochem J. 1974 Nov;144(2):319-25. doi: 10.1042/bj1440319.

DOI:10.1042/bj1440319
PMID:4462585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168499/
Abstract

Resealed chromaffin-granule ;ghosts' were used to study the steady-state kinetics of catecholamine transport. The pump has a high affinity for (-)-noradrenaline, (-)-adrenaline, tyramine and 5-hydroxytryptamine (serotonin), but a lower affinity for (+)-noradrenaline. The measured rates of incorporation do not conform to Michaelis-Menten kinetics, but affinity constants for the former substrates are in the range 8-18mum. Reserpine is a potent inhibitor. Incorporation as a function of ATP concentration also fails to show simple kinetics; the affinity constant for ATP is deduced to be about 3mm at 1mm-MgCl(2). Adenylyl (betagamma-methylene)diphosphonate is a competitive inhibitor at low concentrations, but inhibits more strongly at high concentrations. The pump has a transition temperature at 29 degrees C and does not seem to be identical with the Mg(2+)-stimulated adenosine triphosphatase of chromaffin granules.

摘要

重新封闭的嗜铬颗粒“空壳”被用于研究儿茶酚胺转运的稳态动力学。该泵对（-）-去甲肾上腺素、（-）-肾上腺素、酪胺和5-羟色胺（血清素）具有高亲和力，但对（+）-去甲肾上腺素的亲和力较低。测得的摄取速率不符合米氏动力学，但前几种底物的亲和常数在8 - 18μm范围内。利血平是一种强效抑制剂。作为ATP浓度函数的摄取也未表现出简单的动力学；在1mmol/L MgCl₂ 条件下，ATP的亲和常数推断约为3mmol/L。腺苷（βγ-亚甲基）二膦酸盐在低浓度时是竞争性抑制剂，但在高浓度时抑制作用更强。该泵的转变温度为29℃，似乎与嗜铬颗粒的Mg²⁺ 刺激的三磷酸腺苷酶不同。

相似文献

1
Steady-state kinetics of catecholamine transport by chromaffin-granule "ghosts".嗜铬粒蛋白“空壳”转运儿茶酚胺的稳态动力学
Biochem J. 1974 Nov;144(2):319-25. doi: 10.1042/bj1440319.
2
Ghosts of chromaffin granules accumulate biogenic amines according to a "pump and leak system" without contribution of carrier-mediated efflux.嗜铬粒蛋白的“幽灵”根据“泵-漏系统”积累生物胺，而不依赖载体介导的流出作用。
Naunyn Schmiedebergs Arch Pharmacol. 1990 Sep;342(3):312-22. doi: 10.1007/BF00169443.
3
Transport of catecholamines by resealed chromaffin-grnaule "ghosts".儿茶酚胺通过重封的嗜铬颗粒“空壳”的转运。
Biochem J. 1974 Nov;144(2):311-8. doi: 10.1042/bj1440311.
4
Reserpine as a competitive and reversible inhibitor of the catecholamine transporter of bovine chromaffin granules.利血平作为牛嗜铬颗粒儿茶酚胺转运体的竞争性可逆抑制剂。
FEBS Lett. 1979 Apr 1;100(1):175-8. doi: 10.1016/0014-5793(79)81158-8.
5
Adenosine triphosphate in the bovine chromaffin granule.牛嗜铬颗粒中的三磷酸腺苷
J Physiol (Paris). 1978;74(5):503-8.
6
Catecholamine content of chromaffin granule "ghosts' isolated from bovine adrenal glands.从牛肾上腺分离出的嗜铬粒“空泡”中的儿茶酚胺含量
Biochim Biophys Acta. 1982 Feb 25;714(3):471-8. doi: 10.1016/0304-4165(82)90156-8.
7
ATP-activated exchange of catecholamines by isolated intact chromaffin granules.分离的完整嗜铬颗粒中ATP激活的儿茶酚胺交换
Fed Proc. 1982 Sep;41(11):2755-8.
8
Mechanisms of accumulation of tyramine, metaraminol, and isoproterenol in isolated chromaffin granules and ghosts.酪胺、间羟胺和异丙肾上腺素在分离的嗜铬粒和空泡中的蓄积机制。
Biochem Pharmacol. 1982 Mar 1;31(5):815-23. doi: 10.1016/0006-2952(82)90468-3.
9
Inhibition of catecholamine transport into chromaffin granule ghosts isolated from bovine adrenal glands by phenytoin.苯妥英对从牛肾上腺分离的嗜铬粒蛋白幽灵中儿茶酚胺转运的抑制作用。
J Pharmacol Exp Ther. 1984 Jul;230(1):171-4.
10
Coupling of H+ gradients to catecholamine transport in chromaffin granules.嗜铬颗粒中H⁺梯度与儿茶酚胺转运的偶联。
Ann N Y Acad Sci. 1985;456:254-67. doi: 10.1111/j.1749-6632.1985.tb14874.x.

引用本文的文献

1
Characterization of the monoamine carrier of chromaffin granule membrane by binding of [2-3H]dihydrotetrabenazine.通过[2-³H]二氢丁苯那嗪结合对嗜铬粒细胞膜单胺载体的表征
Proc Natl Acad Sci U S A. 1983 Jan;80(2):584-8. doi: 10.1073/pnas.80.2.584.
2
5-Hydroxytryptamine transport in cells and secretory granules from a transplantable rat insulinoma.来自可移植大鼠胰岛素瘤的细胞和分泌颗粒中的5-羟色胺转运
Biochem J. 1983 Mar 15;210(3):803-10. doi: 10.1042/bj2100803.
3
Nucleotide and bivalent cation specificity of the insulin-granule proton translocase.胰岛素颗粒质子转运体的核苷酸和二价阳离子特异性
Biochem J. 1983 Jan 15;210(1):235-42. doi: 10.1042/bj2100235.
4
Fast axonal transport of foreign transmitters in an identified serotonergic neurone of Aplysia californica.外来递质在加州海兔一个已鉴定的5-羟色胺能神经元中的快速轴突运输。
J Physiol. 1980 Oct;307:259-72. doi: 10.1113/jphysiol.1980.sp013434.
5
Transport of catecholamines by resealed chromaffin-grnaule "ghosts".儿茶酚胺通过重封的嗜铬颗粒“空壳”的转运。
Biochem J. 1974 Nov;144(2):311-8. doi: 10.1042/bj1440311.
6
Effects of the sulfhydryl reagent N-ethylmaleimide on reserpine binding to the catecholamine transporter in chromaffin granule membranes.巯基试剂N-乙基马来酰亚胺对利血平与嗜铬粒细胞膜中儿茶酚胺转运体结合的影响。
Cell Mol Neurobiol. 1988 Jun;8(2):217-24. doi: 10.1007/BF00711247.
7
A sodium/proton antiporter in chromaffin-granule membranes.嗜铬粒膜中的一种钠/质子反向转运体。
Biochem J. 1989 Jan 15;257(2):499-507. doi: 10.1042/bj2570499.
8
Ghosts of chromaffin granules accumulate biogenic amines according to a "pump and leak system" without contribution of carrier-mediated efflux.嗜铬粒蛋白的“幽灵”根据“泵-漏系统”积累生物胺，而不依赖载体介导的流出作用。
Naunyn Schmiedebergs Arch Pharmacol. 1990 Sep;342(3):312-22. doi: 10.1007/BF00169443.
9
A potassium ion diffusion potential causes adrenaline uptake in chromaffin-granule 'ghosts'.钾离子扩散电位导致嗜铬颗粒“空壳”摄取肾上腺素。
Biochem J. 1979 Jun 15;180(3):579-85. doi: 10.1042/bj1800579.
10
Adenosine triphosphatase and adenosine diphosphate/adenosine triphosphate isotope-exchange activities of the chromaffin-granule membrane.嗜铬粒膜的三磷酸腺苷酶及二磷酸腺苷/三磷酸腺苷同位素交换活性
Biochem J. 1977 Oct 1;167(1):297-300. doi: 10.1042/bj1670297.

本文引用的文献

1
Uptake of catecholamines by a particulate fraction of the adrenal medulla.肾上腺髓质微粒体部分对儿茶酚胺的摄取
J Biol Chem. 1962 Jul;237:2311-7.
2
THE ADENOSINE-TRIPHOSPHATASE ACTIVITY OF ADRENAL CHROMAFFIN GRANULES.肾上腺嗜铬颗粒的三磷酸腺苷酶活性
Biochem J. 1965 May;95(2):490-6. doi: 10.1042/bj0950490.
3
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
4
Sterospecificity of amine uptake mechanism in nerve granules.
J Pharmacol Exp Ther. 1965 Dec;150(3):335-40.
5
Reuptake and net uptake of noradrenaline in adrenergic nerve granules with a note on the affinity for 1- and d-isomers.
Acta Physiol Scand. 1967 Oct-Nov;71(2):151-62. doi: 10.1111/j.1748-1716.1967.tb03721.x.
6
Negative cooperativity in regulatory enzymes.调节酶中的负协同性。
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1121-8. doi: 10.1073/pnas.62.4.1121.
7
Active sodium and potassium transport in high potassium and low potassium sheep red cells.高钾和低钾绵羊红细胞中的钠钾主动转运
J Gen Physiol. 1971 Oct;58(4):438-66. doi: 10.1085/jgp.58.4.438.
8
Half-of-the-sites reactivity and the conformational states of cytidine triphosphate synthetase.三磷酸胞苷合成酶的半数位点反应性与构象状态
Biochemistry. 1971 Aug 31;10(18):3371-8. doi: 10.1021/bi00794a009.
9
Compartmental analysis of amine storage in bovine adrenal medullary granules.牛肾上腺髓质颗粒中胺储存的区室分析。
Mol Pharmacol. 1971 May;7(3):308-16.
10
The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli.磷酸烯醇丙酮酸-磷酸转移酶系统在大肠杆菌分离膜制剂转运糖类中的作用。
J Biol Chem. 1968 Jul 10;243(13):3711-24.