嗜铬粒蛋白“空壳”转运儿茶酚胺的稳态动力学
Steady-state kinetics of catecholamine transport by chromaffin-granule "ghosts".
作者信息
Phillips J H
出版信息
Biochem J. 1974 Nov;144(2):319-25. doi: 10.1042/bj1440319.
Abstract
Resealed chromaffin-granule ;ghosts' were used to study the steady-state kinetics of catecholamine transport. The pump has a high affinity for (-)-noradrenaline, (-)-adrenaline, tyramine and 5-hydroxytryptamine (serotonin), but a lower affinity for (+)-noradrenaline. The measured rates of incorporation do not conform to Michaelis-Menten kinetics, but affinity constants for the former substrates are in the range 8-18mum. Reserpine is a potent inhibitor. Incorporation as a function of ATP concentration also fails to show simple kinetics; the affinity constant for ATP is deduced to be about 3mm at 1mm-MgCl(2). Adenylyl (betagamma-methylene)diphosphonate is a competitive inhibitor at low concentrations, but inhibits more strongly at high concentrations. The pump has a transition temperature at 29 degrees C and does not seem to be identical with the Mg(2+)-stimulated adenosine triphosphatase of chromaffin granules.
摘要
重新封闭的嗜铬颗粒“空壳”被用于研究儿茶酚胺转运的稳态动力学。该泵对（-）-去甲肾上腺素、（-）-肾上腺素、酪胺和5-羟色胺（血清素）具有高亲和力，但对（+）-去甲肾上腺素的亲和力较低。测得的摄取速率不符合米氏动力学，但前几种底物的亲和常数在8 - 18μm范围内。利血平是一种强效抑制剂。作为ATP浓度函数的摄取也未表现出简单的动力学；在1mmol/L MgCl₂ 条件下，ATP的亲和常数推断约为3mmol/L。腺苷（βγ-亚甲基）二膦酸盐在低浓度时是竞争性抑制剂，但在高浓度时抑制作用更强。该泵的转变温度为29℃，似乎与嗜铬颗粒的Mg²⁺ 刺激的三磷酸腺苷酶不同。
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