Ambler R P
Biochem J. 1973 Dec;135(4):751-8. doi: 10.1042/bj1350751.
The amino acid sequence of the cytochrome c' from Alcaligenes sp. N.C.I.B. 11015 (Iwasaki's ;Pseudomonas denitrificans') has been determined. This organism is the only non-photosynthetic bacterium in which the protein has been found. The protein consists of a single polypeptide chain of 127 residues, with a single haem covalently attached to two cysteines. Unlike normal cytochromes c, the haem attachment site is very close to the C-terminus. The amino acid sequence around the haem attachment site is very similar to that of Chromatium vinosum D cytochrome c'. Detailed evidence for the amino acid sequence of the protein has been deposited as Supplementary Publication SUP 50022 at the British Library (Lending Division), (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5.
已确定产碱杆菌属(N.C.I.B. 11015,即岩崎氏菌;反硝化假单胞菌)细胞色素c'的氨基酸序列。该微生物是唯一一种发现含有这种蛋白质的非光合细菌。该蛋白质由一条含127个残基的单多肽链组成,单个血红素通过共价键与两个半胱氨酸相连。与正常细胞色素c不同,血红素连接位点非常靠近C端。血红素连接位点周围的氨基酸序列与嗜硫红假单胞菌D细胞色素c'非常相似。该蛋白质氨基酸序列的详细证据已作为补充出版物SUP 50022存放在英国图书馆(借阅部)(原国家科学技术借阅图书馆),地址为英国约克郡波士顿温泉市LS23 7BQ,可按《生物化学杂志》(1973年)第131卷第5期给出的条件从该处获取复印件。