一种用于测定血液中L-麦角硫因的简便分光光度法。
A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.
作者信息
Carlsson J, Kierstan M P, Brocklehurst K
出版信息
Biochem J. 1974 Apr;139(1):237-42. doi: 10.1042/bj1390237.
Abstract
- A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu(2+)-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these conditions) by its virtually instantaneous reaction with 2,2'-dipyridyl disulphide at pH1. 3. This method and the results obtained with it for the analysis of human, horse, sheep and pig blood are compared with existing methods of l-ergothioneine analysis and the results obtained thereby.
摘要
- 开发了一种简便的分光光度法，用于测定溶液中的L-麦角硫因，包括去蛋白的血液溶血产物。2. 该方法包括通过热沉淀进行去蛋白，以及在碱性介质中铜（2+）催化的谷胱甘肽和L-抗坏血酸等硫醇的氧化，然后在pH1条件下，通过L-麦角硫因与2,2'-二吡啶二硫化物几乎瞬间反应来滴定L-麦角硫因（在这些条件下它不会被氧化）。3. 将该方法及其用于分析人、马、羊和猪血所得到的结果，与现有的L-麦角硫因分析方法及其所得到的结果进行了比较。
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A convenient spectrophotometric assay for the determination of L-ergothioneine in blood.一种用于测定血液中L-麦角硫因的简便分光光度法。
Biochem J. 1974 Apr;139(1):237-42. doi: 10.1042/bj1390237.
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Biochem J. 1974 Apr;139(1):221-35. doi: 10.1042/bj1390221.
本文引用的文献
1
A new test for ergothioneine upon which is based a method for its estimation in simple solution and in blood-filtrates.一种新的麦角硫因检测方法，基于该方法可对其在简单溶液和血液滤液中的含量进行测定。
Biochem J. 1928;22(1):4-10. doi: 10.1042/bj0220004.
2
The determination of ergothioneine; the non-occurrence of ergothioneine in urine.麦角硫因的测定；尿液中未检测到麦角硫因。
Biochem J. 1950 Nov-Dec;47(5):513-8. doi: 10.1042/bj0470513.
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Specificity and mechanism of the Hunter reaction for ergothioneine.麦角硫因的亨特反应的特异性及机制。
Nature. 1951 Jan 13;167(4237):82-3. doi: 10.1038/167082a0.
4
[Occurrence of herzynine together with ergothioneine in boar ejaculate and cattle erythrocytes and the biological relationship of the two bases].[猪精液和牛红细胞中肌肽与麦角硫因的共存情况以及两种碱基的生物学关系]
Hoppe Seylers Z Physiol Chem. 1959;314(1):33-7.
5
The distribution of ergothioneine in blood as determined by a new method of estimation.通过一种新的估算方法测定血液中麦角硫因的分布。
Biochem J. 1958 Dec;70(4):656-60. doi: 10.1042/bj0700656.
6
The preparation and properties of ergothioneine disulphide.二硫代麦角硫因的制备及其性质
Biochem J. 1958 Feb;68(2):204-10. doi: 10.1042/bj0680204.
7
The biosynthesis of ergothioneine and histidine by Claviceps purpurea. I. The incorporation of [2-14C]acetate.麦角菌合成麦角硫因和组氨酸。I. [2-¹⁴C]乙酸盐的掺入。
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