豌豆子叶中的蛋白质合成。参与苯丙氨酰转移核糖核酸与核糖体结合的蛋白质因子。
Protein synthesis in the cotyledons of Pisum sativum L. Protein factors involved in the binding of phenylalanyl-transfer ribonucleic acid to ribosomes.
作者信息
Wells G N, Beevers L
出版信息
Biochem J. 1974 Apr;139(1):61-9. doi: 10.1042/bj1390061.
Abstract
- Proteinaceous factors contained in a 0.5m-KCl extract of ribosomes from pea cotyledons form a ternary complex at 0 degrees C with [(14)C]phenylalanyl-tRNA and poly(U). The complex is measured by its quantitative retention on Millipore filters. 2. Complex-assembly is optimal at 5mm-Mg(2+) and is independent of GTP and ribosomes. 3. The addition of ribosomes is required to stabilize the complex at 34 degrees C. The complex binds to a puromycin-sensitive site on the ribosome. 4. Soluble factors from the 250000g supernatant of pea cotyledon form a Millipore-retainable complex dependent on GTP and ribosomes. 5. Complex-formation by soluble factors has a Mg(2+) optimum of 10-12mm and forms a puromycin-insensitive complex with ribosomes. 6. The function of the ribosomal protein factors and the supernatant fraction in initiation of protein synthesis is discussed.
摘要
- 豌豆子叶核糖体的0.5m - KCl提取物中所含的蛋白质因子在0℃时与[¹⁴C]苯丙氨酰 - tRNA和聚（U）形成三元复合物。该复合物通过其在微孔滤膜上的定量保留来测定。2. 复合物组装在5mm - Mg²⁺时最佳，且不依赖于GTP和核糖体。3. 需要添加核糖体以在34℃稳定复合物。该复合物与核糖体上的嘌呤霉素敏感位点结合。4. 豌豆子叶250000g上清液中的可溶性因子形成依赖于GTP和核糖体的可被微孔滤膜保留的复合物。5. 可溶性因子形成复合物的最佳Mg²⁺浓度为10 - 12mm，并与核糖体形成对嘌呤霉素不敏感的复合物。6. 讨论了核糖体蛋白质因子和上清液组分在蛋白质合成起始中的作用。
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Protein Synthesis in Cotyledons of Pisum sativum L: I. Changes in Cell-Free Amino Acid Incorporation Capacity during Seed Development and Maturation.豌豆子叶的蛋白质合成：I. 种子发育和成熟过程中无细胞氨基酸掺入能力的变化。
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