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1
Formation of a mammalian initiation complex with reovirus messenger RNA, methionyl-tRNA F , and ribosomal subunits.呼肠孤病毒信使核糖核酸、甲硫氨酰 - tRNA F和核糖体亚基形成哺乳动物起始复合物。
Proc Natl Acad Sci U S A. 1972 May;69(5):1234-8. doi: 10.1073/pnas.69.5.1234.
2
Polypeptide chain initiation and stepwise elongation with Artemia ribosomes and factors.卤虫核糖体及因子参与的多肽链起始与逐步延伸过程
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2451-5. doi: 10.1073/pnas.69.9.2451.
3
Preparation and characterization of eukaryotic initiation factor EIF-3. Formation of binary (EIF-3-Met-tRNAf) and ternary (EIF-3-Met-tRNAf-GTP) complexes.真核起始因子EIF-3的制备与表征。二元复合物（EIF-3-甲硫氨酰-tRNAf）和三元复合物（EIF-3-甲硫氨酰-tRNAf-GTP）的形成。
J Biol Chem. 1976 Apr 10;251(7):1926-35.
4
Studies on native ribosomal subunits from rat liver. Evidence for activities associated with native 40S subunits that affect the interaction with acetylphenylalanyl-tRNA, methionyl-tRNAf, and 60S subunits.对大鼠肝脏天然核糖体亚基的研究。与天然40S亚基相关的活性影响其与乙酰苯丙氨酰 - tRNA、甲硫氨酰 - tRNAf及60S亚基相互作用的证据。
Biochemistry. 1975 Dec 2;14(24):5328-35. doi: 10.1021/bi00695a017.
5
The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。
Eur J Biochem. 1976 Feb 16;62(2):243-55. doi: 10.1111/j.1432-1033.1976.tb10154.x.
6
Requirement for GTP in the initiation process on reticulocyte ribosomes and ribosomal subunits.网织红细胞核糖体及核糖体亚基起始过程中对鸟苷三磷酸（GTP）的需求。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2246-51. doi: 10.1073/pnas.68.9.2246.
7
Protein synthesis in rabbit reticulocytes. A study of Met-tRNA f Met binding factor(s) and Met-tRNA f Met binding to ribosomes and AUG codon.兔网织红细胞中的蛋白质合成。对甲硫氨酰 - tRNAfMet 结合因子及甲硫氨酰 - tRNAfMet 与核糖体和 AUG 密码子结合的研究。
J Biol Chem. 1975 Feb 10;250(3):853-62.
8
Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.真核生物中的蛋白质起始：由部分纯化的核糖体因子、甲硫氨酰转移RNA和三磷酸鸟苷组成的三元复合物的形成与功能。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):41-5. doi: 10.1073/pnas.70.1.41.
9
Initial dipeptide formation in hemoglobin biosynthesis.血红蛋白生物合成中的初始二肽形成。
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1810-4. doi: 10.1073/pnas.68.8.1810.
10
Initiation of hemoglobin synthesis: comparison of model reactions that use artificial templates with those using natural messenger RNA.血红蛋白合成的起始：使用人工模板的模型反应与使用天然信使核糖核酸的模型反应的比较。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):706-11. doi: 10.1073/pnas.69.3.706.

引用本文的文献

1
Mammalian orthoreovirus particles induce and are recruited into stress granules at early times postinfection.哺乳动物正呼肠孤病毒颗粒在感染后早期诱导并被募集到应激颗粒中。
J Virol. 2009 Nov;83(21):11090-101. doi: 10.1128/JVI.01239-09. Epub 2009 Aug 26.
2
Use of purified polysomes from rabbit reticulocytes in a specific test for initiation factors.在一项针对起始因子的特定测试中使用来自兔网织红细胞的纯化多核糖体。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):68-72. doi: 10.1073/pnas.71.1.68.
3
Protein initiation in eukaryotes: formation and function of a ternary complex composed of a partially purified ribosomal factor, methionyl transfer RNA, and guanosine triphosphate.真核生物中的蛋白质起始：由部分纯化的核糖体因子、甲硫氨酰转移RNA和三磷酸鸟苷组成的三元复合物的形成与功能。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):41-5. doi: 10.1073/pnas.70.1.41.
4
On the mechanism of delayed inhibition of protein synthesis in heme-defecient rabbit reticulocyte lysates.关于血红素缺乏的兔网织红细胞裂解物中蛋白质合成延迟抑制的机制
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3506-10. doi: 10.1073/pnas.73.10.3506.

本文引用的文献

1
RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
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Reovirus: RNA polymerase activity in purified virions.呼肠孤病毒：纯化病毒颗粒中的RNA聚合酶活性
Biochem Biophys Res Commun. 1968 Dec 30;33(6):895-901. doi: 10.1016/0006-291x(68)90396-3.
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Sequence at the 5'-end of bacteriophage f2 RNA.噬菌体f2 RNA 5'-末端的序列
Biochem Biophys Res Commun. 1971 Jan 8;42(1):82-8. doi: 10.1016/0006-291x(71)90365-2.
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Initiation of protein synthesis in eukaryotes.真核生物中蛋白质合成的起始
Biochem Biophys Res Commun. 1971 Feb 5;42(3):462-8. doi: 10.1016/0006-291x(71)90393-7.
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Messenger activity in mammalian cell-free extracts of reovirus single-stranded RNA prepared in vitro.体外制备的呼肠孤病毒单链RNA在哺乳动物无细胞提取物中的信使活性。
Biochem Biophys Res Commun. 1971 Feb 5;42(3):454-61. doi: 10.1016/0006-291x(71)90392-5.
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Protein chain initiation by methionyl-tRNA.甲硫氨酰 - tRNA引发蛋白质链起始。
Biochem Biophys Res Commun. 1970 Jul 27;40(2):416-21. doi: 10.1016/0006-291x(70)91025-9.
8
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Nature. 1970 Aug 29;227(5261):918-20. doi: 10.1038/227918a0.
9
Transient incorporation of methionine at the N-terminus of protamine newly synthesized in trout testis cells.甲硫氨酸在鲑鱼睾丸细胞中新合成的鱼精蛋白N端的瞬时掺入。
Nature. 1970 Aug 15;227(5259):676-80. doi: 10.1038/227676a0.
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Role of methionine in the initiation of haemoglobin synthesis.蛋氨酸在血红蛋白合成起始过程中的作用。
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呼肠孤病毒信使核糖核酸、甲硫氨酰 - tRNA F和核糖体亚基形成哺乳动物起始复合物。

Formation of a mammalian initiation complex with reovirus messenger RNA, methionyl-tRNA F , and ribosomal subunits.

作者信息

Levin D H, Kyner D, Acs G

出版信息

Proc Natl Acad Sci U S A. 1972 May;69(5):1234-8. doi: 10.1073/pnas.69.5.1234.

DOI:10.1073/pnas.69.5.1234
PMID:4504335
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC426671/
Abstract

Previous data demonstrated that reovirus mRNA, synthesized in vitro with the particulate RNA transcriptase of reovirus cores, efficiently directs the synthesis of polypeptides in vitro. The present studies indicate that all of the three size classes of reovirus mRNA produced in vitro can form protein initiation complexes with rat liver [(36)S]Met-tRNA(F) and incubated 40S and 60S ribosomal subunits, which had been washed in 0.5 M KCl of mouse fibroblast L-929 cells. Mild prior treatment of the mRNA with HCHO was required to expose the initiator region. The initiation complex reacted quantitatively with puromycin to form a puromycin peptide, whose electrophoretic properties were identical to methionyl-puromycin formed in response to poly(A,G,U) or the initiator codon AUG. The complex was relatively stable and specific for [(35)S]Met-tRNA(F); rat liver [(35)S]Met-tRNA(M) was unreactive unless the supernatant factors EF T(1) and EF T(2) were also present. However, the addition of fusidic acid, at a concentration that did not affect complex formation with [(35)S]Met-tRNA(F), completely inhibited Met-tRNA(M) utilization. Exogenous ribosomal factors and GTP were not required unless the separated 40S and 60S subunits were further treated with 1 M KCl. The data suggest that reovirus mRNA contains AUG initiator codons that form a complex with Met-tRNA(F) at a puromycin-reactive site on ribosomes.

摘要

先前的数据表明，用呼肠孤病毒核心的颗粒状RNA转录酶在体外合成的呼肠孤病毒mRNA能在体外有效地指导多肽的合成。目前的研究表明，体外产生的所有三种大小类别的呼肠孤病毒mRNA都能与大鼠肝脏[（36）S]甲硫氨酰 - tRNA（F）以及经小鼠成纤维细胞L - 929细胞的0.5M KCl洗涤过的40S和60S核糖体亚基形成蛋白质起始复合物。mRNA需先用甲醛进行温和预处理以暴露起始区域。起始复合物与嘌呤霉素定量反应形成嘌呤霉素肽，其电泳性质与响应聚（A，G，U）或起始密码子AUG形成的甲硫氨酰 - 嘌呤霉素相同。该复合物相对稳定且对[（35）S]甲硫氨酰 - tRNA（F）具有特异性；大鼠肝脏[（35）S]甲硫氨酰 - tRNA（M）无反应，除非还存在上清液因子EF T（1）和EF T（2）。然而，添加浓度不影响与[（35）S]甲硫氨酰 - tRNA（F）形成复合物的夫西地酸，会完全抑制甲硫氨酰 - tRNA（M）的利用。除非分离的40S和60S亚基进一步用1M KCl处理，否则不需要外源核糖体因子和GTP。数据表明，呼肠孤病毒mRNA含有AUG起始密码子，这些密码子在核糖体上的嘌呤霉素反应位点与甲硫氨酰 - tRNA（F）形成复合物。